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Environ Technol

Title:		Isolation and characterization of novel bacterial strain present in a lab scale hybrid UASB reactor treating distillery spent wash
Author(s):		Thiyagu R; Sivarajan P;
Address:		"Department of Civil Engineering, Annamalai University , Annamalai Nagar , India"
Journal Title:		Environ Technol
Year:		2019
Volume:		20180515
Issue:		25
Page Number:		3351 - 3357
DOI:		10.1080/09593330.2018.1473499
ISSN/ISBN:		1479-487X (Electronic) 0959-3330 (Linking)
Abstract:		"Recalcitrant compounds degrading novel bacteria was isolated from lab scale hybrid UASB reactor treating distillery spent wash, enriched with biomass activity. The granules were subjected to SEM analysis to classify and isolate the bacterial strains. The strain was ubiquitous, mesophilic, gram-negative, motile, non-spore forming and cultivable optimally at 30 degrees C at pH 7. Most potential isolates from the strains were subjected to 16S rRNA sequencing and branded as a member of diverse genera, gamma-proteobacteria, Stenotrophomonas sp. Based on 16S rRNA sequencing, genomic DNA extraction, PCR amplification, and phylogeny analysis the gram-negative bacteria was identified as Stenotrophomonas maltophilia and found to degrade distillery spent wash. The hybrid UASB reactor was operated for 360 days with 24 h HRT and has an optimum COD removal efficiency of 83.87% at an organic loading rates (OLRs) ranging within 0.25-27.40 kg COD/m(3 )d. Abbreviations: 16S rRNA: 16S ribosomal RNA; COD: Chemical oxygen demand; DNA: Deoxyribonucleic acid; dS/m: deciSiemens per metre; g/L: gram per litre; HRT: Hydraulic retention time; mg/L: milligram per litre; OLR: Organic loading rate; PCR: Polymerase chain reaction; RNA: Ribonucleic acid; UASB: Upflow anaerobic sludge blanket; VFA: Volatile fatty acid; VSS: Volatile suspended solid"
Keywords:		"Anaerobiosis *Bioreactors RNA, Ribosomal, 16S Sewage *Waste Disposal, Fluid 16S rRNA gene sequence distillery spent wash hybrid UASB reactor recalcitrant compounds;"
Notes:		"MedlineThiyagu, R Sivarajan, P eng England 2018/05/05 Environ Technol. 2019 Nov; 40(25):3351-3357. doi: 10.1080/09593330.2018.1473499. Epub 2018 May 15"