« Previous Abstract"Emission characteristics, environmental impact assessment and priority control strategies derived from VOCs speciation sourcely through measurement for wooden furniture-manufacturing industry in China"    Next Abstract"Histone acetylation, chromatin remodelling, transcription and nucleotide excision repair in S. cerevisiae: studies with two model genes" »

Nucleic Acids Res

Title:		The mapping of nucleosomes and regulatory protein binding sites at the Saccharomyces cerevisiae MFA2 gene: a high resolution approach
Author(s):		Teng Y; Yu S; Waters R;
Address:		"School of Biological Sciences, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK"
Journal Title:		Nucleic Acids Res
Year:		2001
Volume:		29
Issue:		13
Page Number:		E64 - E64
DOI:		10.1093/nar/29.13.e64
ISSN/ISBN:		1362-4962 (Electronic) 0305-1048 (Print) 0305-1048 (Linking)
Abstract:		We have developed an end-labelling approach to map the positions of nucleosomes and protein binding sites at nucleotide resolution by footprinting micrococcal nuclease (MNase)-sensitive sites. Using this approach we determined that the MFA2 gene and its upstream control regions have four positioned nucleosomes when transcription is repressed in mating type alpha cells and that the nucleosomes lose their positioning when the gene became transcriptionally active in mating type a cells. We also detected MNase-hypersensitive sites in the alpha2 operator region of MFA2 in alpha cells but not in a cells. These probably result from the change in the local DNA conformation due to protein(s) binding in this region that governs MFA2 transcription
Keywords:		"Base Sequence Binding Sites DNA Footprinting/*methods DNA Probes DNA, Fungal/chemistry/genetics/metabolism DNA-Binding Proteins/metabolism Fungal Proteins/genetics/metabolism Gene Expression Regulation, Fungal Gene Silencing Genes, Fungal/genetics Genes, ;"
Notes:		"MedlineTeng, Y Yu, S Waters, R eng Research Support, Non-U.S. Gov't England 2001/07/04 Nucleic Acids Res. 2001 Jul 1; 29(13):E64-4. doi: 10.1093/nar/29.13.e64"