| Title: | Adaptation of hydroxymethylbutenyl diphosphate reductase enables volatile isoprenoid production |
| Author(s): | Bongers M; Perez-Gil J; Hodson MP; Schrubbers L; Wulff T; Sommer MO; Nielsen LK; Vickers CE; |
| Address: | "Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark. Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Australia. Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus UAB Bellaterra, Barcelona, Spain. Metabolomics Australia, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Australia. School of Pharmacy, The University of Queensland, Brisbane, Australia. CSIRO Synthetic Biology Future Science Platform, Brisbane, Australia" |
| ISSN/ISBN: | 2050-084X (Electronic) 2050-084X (Linking) |
| Abstract: | "Volatile isoprenoids produced by plants are emitted in vast quantities into the atmosphere, with substantial effects on global carbon cycling. Yet, the molecular mechanisms regulating the balance between volatile and non-volatile isoprenoid production remain unknown. Isoprenoids are synthesised via sequential condensation of isopentenyl pyrophosphate (IPP) to dimethylallyl pyrophosphate (DMAPP), with volatile isoprenoids containing fewer isopentenyl subunits. The DMAPP:IPP ratio could affect the balance between volatile and non-volatile isoprenoids, but the plastidic DMAPP:IPP ratio is generally believed to be similar across different species. Here we demonstrate that the ratio of DMAPP:IPP produced by hydroxymethylbutenyl diphosphate reductase (HDR/IspH), the final step of the plastidic isoprenoid production pathway, is not fixed. Instead, this ratio varies greatly across HDRs from phylogenetically distinct plants, correlating with isoprenoid production patterns. Our findings suggest that adaptation of HDR plays a previously unrecognised role in determining in vivo carbon availability for isoprenoid emissions, directly shaping global biosphere-atmosphere interactions" |
| Keywords: | Acclimatization Gene Knockdown Techniques Metabolic Networks and Pathways Metabolomics/methods Oxidoreductases/genetics/*metabolism Phylogeny Plants/classification/genetics/*metabolism Proteomics/methods Terpenes/*metabolism Volatile Organic Compounds/*me; |
| Notes: | "MedlineBongers, Mareike Perez-Gil, Jordi Hodson, Mark P Schrubbers, Lars Wulff, Tune Sommer, Morten Oa Nielsen, Lars K Vickers, Claudia E eng DP140103514/Australian Research Council/ NNF10CC1016517/Novo Nordisk Foundation/ FP7-PEOPLE-2013-IOF. Project: 623679/Marie Sklodowska-Curie Actions/ Research Support, Non-U.S. Gov't England 2020/03/13 Elife. 2020 Mar 12; 9:e48685. doi: 10.7554/eLife.48685" |
