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J Agric Food Chem

Title:		Identification of volatile markers in potato brown rot and ring rot by combined GC-MS and PTR-MS techniques: study on in vitro and in vivo samples
Author(s):		Blasioli S; Biondi E; Samudrala D; Spinelli F; Cellini A; Bertaccini A; Cristescu SM; Braschi I;
Address:		"Department of Agricultural Sciences, University of Bologna , Viale G. Fanin 44, 40127 Bologna, Italy"
Journal Title:		J Agric Food Chem
Year:		2014
Volume:		20131230
Issue:		2
Page Number:		337 - 347
DOI:		10.1021/jf403436t
ISSN/ISBN:		1520-5118 (Electronic) 0021-8561 (Linking)
Abstract:		"Ralstonia solanacearum (Rs) and Clavibacter michiganensis subsp. sepedonicus (Cms) are the bacterial causal agents of potato brown and ring rot, respectively, and are included in the A2 list of quarantine pathogens in Europe. Identification by GC-MS analysis of volatile organic compounds from Rs or Cms cultured on different nutrient media was performed. GC-MS and PTR-MS analysis were carried out also on unwounded potato tubers infected with the same pathogens. Infected tubers were produced by experimental inoculations of the plants. In in vitro experiments, Rs or Cms emitted volatile compounds, part of which were specific disease markers of potato (2-propanol and 3-methylbutanoic acid), mainly originating from bacterial metabolism (i.e., amino acid degradation, carbohydrate and fatty acid oxidation). In potato tubers, pathogen metabolism modified the volatile compound pattern emitted from healthy samples. Both bacteria seem to accelerate metabolic processes ongoing in potatoes and, in the case of Rs, disease markers (1-hepten-3-ol, 3,6-dimethyl-3-octanone, 3-ethyl-3-methylpentane, 1-chloroctane, and benzothiazole) were identified"
Keywords:		2-Propanol/analysis Actinobacteria/metabolism Biomarkers/*analysis Gas Chromatography-Mass Spectrometry/*methods Hemiterpenes Mass Spectrometry/*methods Pentanoic Acids/analysis Plant Diseases/*microbiology Plant Tubers/chemistry/metabolism/microbiology R;
Notes:		"MedlineBlasioli, Sonia Biondi, Enrico Samudrala, Devasena Spinelli, Francesco Cellini, Antonio Bertaccini, Assunta Cristescu, Simona M Braschi, Ilaria eng Research Support, Non-U.S. Gov't 2013/12/10 J Agric Food Chem. 2014 Jan 15; 62(2):337-47. doi: 10.1021/jf403436t. Epub 2013 Dec 30"