| Title: | Determination of ketones and ethyl acetate-a preliminary study for the discrimination of patients with lung cancer |
| Author(s): | Santos PM; del Nogal Sanchez M; Pozas APC; Pavon JLP; Cordero BM; |
| Address: | "Departamento de Quimica Analitica, Nutricion y Bromatologia, Facultad de Ciencias Quimicas, Universidad de Salamanca, 37008, Salamanca, Spain. Departamento de Quimica Analitica, Nutricion y Bromatologia, Facultad de Ciencias Quimicas, Universidad de Salamanca, 37008, Salamanca, Spain. mns@usal.es. Servicio de Medicina Interna, Hospital Virgen de la Vega, Complejo Asistencial Universitario de Salamanca, 37007, Salamanca, Spain" |
| DOI: | 10.1007/s00216-017-0508-2 |
| ISSN/ISBN: | 1618-2650 (Electronic) 1618-2642 (Linking) |
| Abstract: | "In this work, ten possible volatile biomarkers of lung cancer (acetone, 2-butanone, ethyl acetate, 2-pentanone, 4-methyl-2-pentanone, 2-hexanone, 3-heptanone, 2-heptanone, 3-octanone, and 2-nonanone) have been analyzed to evaluate their different concentration levels in urine samples from lung cancer patients (n = 12) and healthy controls (n = 12). The volatile compounds were generated with a headspace autosampler and analyzed with a gas chromatograph equipped with a programmed temperature vaporizer and mass spectrometry detector (HS-PTV-GC-MS). With the aim of evaluating the aforementioned differences, a Mann-Whitney U test and box-plots were obtained. Very good discrimination between cancer and control groups was achieved for three (ethyl acetate, 3-heptanone, and 3-octanone) of the ten analytes studied. With a view to assigning samples to the group of healthy or ill individuals, the Wilcoxon signed-rank test has been used. In spite of the small number of urine samples assayed, the results may suggest that the studied compounds could be considered useful tools in order to discern samples and they could be employed as a complementary test in a diagnosis. Graphical abstract Classification of samples (lung cancer patients and controls) with the Wilcoxon signed rank test" |
| Keywords: | "Acetates/*urine Aged Aged, 80 and over Biomarkers, Tumor/urine Female Gas Chromatography-Mass Spectrometry/*methods Humans Ketones/*urine Limit of Detection Lung Neoplasms/*urine Male Middle Aged Volatile Organic Compounds/*urine Lung cancer Mass spectrom;" |
| Notes: | "MedlineSantos, Patricia Martin Del Nogal Sanchez, Miguel Pozas, Angel Pedro Crisolino Pavon, Jose Luis Perez Cordero, Bernardo Moreno eng Proyecto SA162U14/Consejeria de Educacion, Junta de Castilla y Leon/ Proyecto CTQ2013-47993-P/BQU/Ministerio de Economia y Competitividad/ Evaluation Study Germany 2017/07/19 Anal Bioanal Chem. 2017 Sep; 409(24):5689-5696. doi: 10.1007/s00216-017-0508-2. Epub 2017 Jul 17" |
