| Title: | Epimerisation of chiral hydroxylactones by short-chain dehydrogenases/reductases accounts for sex pheromone evolution in Nasonia |
| Author(s): | Ruther J; Hagstrom AK; Brandstetter B; Hofferberth J; Bruckmann A; Semmelmann F; Fink M; Lowack H; Laberer S; Niehuis O; Deutzmann R; Lofstedt C; Sterner R; |
| Address: | "Institute of Zoology, University of Regensburg, 93053 Regensburg, Germany. Department of Biology, Lund University, SE-22362 Lund, Sweden. Department of Chemistry, Kenyon College, Gambier, OH 43022, USA. Institute of Biochemistry, Genetics and Microbiology, University of Regensburg, 93053 Regensburg, Germany. Institute of Biophysics and Physical Biochemistry, University of Regensburg, 93053 Regensburg, Germany. Centre for Molecular Biodiversity Research, Zoological Research Museum Alexander Koenig, 53113 Bonn, Germany" |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Males of all species of the parasitic wasp genus Nasonia use (4R,5S)-5-hydroxy-4-decanolide (RS) as component of their sex pheromone while only N. vitripennis (Nv), employs additionally (4R,5R)-5-hydroxy-4-decanolide (RR). Three genes coding for the NAD(+)-dependent short-chain dehydrogenases/reductases (SDRs) NV10127, NV10128, and NV10129 are linked to the ability of Nv to produce RR. Here we show by assaying recombinant enzymes that SDRs from both Nv and N. giraulti (Ng), the latter a species with only RS in the pheromone, epimerise RS into RR and vice versa with (4R)-5-oxo-4-decanolide as an intermediate. Nv-derived SDR orthologues generally had higher epimerisation rates, which were also influenced by NAD(+) availability. Semiquantitative protein analyses of the pheromone glands by tandem mass spectrometry revealed that NV10127 as well as NV10128 and/or NV10129 were more abundant in Nv compared to Ng. We conclude that the interplay of differential expression patterns and SDR epimerisation rates on the ancestral pheromone component RS accounts for the evolution of a novel pheromone phenotype in Nv" |
| Keywords: | "Animals Evolution, Molecular Insect Proteins/genetics/metabolism Lactones/*chemistry Male Oxidoreductases/*genetics/metabolism Pheromones/chemistry/*metabolism Recombinant Proteins/metabolism Sexual Behavior, Animal Tandem Mass Spectrometry Wasps/chemistr;" |
| Notes: | "MedlineRuther, Joachim Hagstrom, Asa K Brandstetter, Birgit Hofferberth, John Bruckmann, Astrid Semmelmann, Florian Fink, Michaela Lowack, Helena Laberer, Sabine Niehuis, Oliver Deutzmann, Rainer Lofstedt, Christer Sterner, Reinhard eng Research Support, Non-U.S. Gov't England 2016/10/06 Sci Rep. 2016 Oct 5; 6:34697. doi: 10.1038/srep34697" |
