| Title: | Breath selection methods for compact mass spectrometry breath analysis |
| Author(s): | Rosenthal K; Ruszkiewicz DM; Allen H; Lindley MR; Turner MA; Hunsicker E; |
| Address: | "School of Sport, Exercise & Health Sciences, Loughborough University, Loughborough, United Kingdom. Translational Chemical Biology Research Group, United Kingdom" |
| ISSN/ISBN: | 1752-7163 (Electronic) 1752-7155 (Linking) |
| Abstract: | "Compact mass spectrometry (CMS) is a versatile and transportable analytical instrument that has the potential to be used in clinical settings to quickly and non-invasively detect a wide range of relevant conditions from breath samples. The purpose of this study is to optimise data preprocessing protocols by three proposed methods of breath sampling, using the CMS. It also lays out a general framework for which data processing methods can be evaluated. METHODS: This paper considers data from three previous studies, each using a different breath sampling method. These include a peppermint washout study using continuous breath sampling with a purified air source, an exercise study using continuous breath sampling with an ambient air source, and a single breath sampling study with an ambient air source. For each dataset, different breath selection (data preprocessing) methods were compared and benchmarked according to predictive performance on a validation set and quantitative reliability of m/z bin intensity measurements. RESULTS: For both continuous methods, the best breath selection method improved the predictive model compared to no preselection, as measured by the 95% CI range for Youden's index, from 0.68-0.86 to 0.86-0.97 for the exercise study and 0.69-0.82 to 1.00-1.00 for the peppermint study. The reliability of intensity measurements for both datasets (as measured by median relative standard deviation (RSD)), was improved slightly by the best selection method compared to no preselection, from 18% to 14% for the exercise study and 7%-5% for the peppermint study. For the single breath samples, all the models resulted in perfect prediction, with a 95% CI range for Youden's index of 1.00-1.00. The reliability of the proposed method was 38%. CONCLUSION: The method of selecting exhaled breath from CMS data can affect the reliability of the measurement and the ability to distinguish between breath samples taken under different conditions. The application of appropriate data processing methods can improve the quality of the data and results obtained from CMS. The methods presented will enable untargeted analysis of breath VOCs using CMS to be performed" |
| Keywords: | "Adolescent Adult Breath Tests/*methods Exercise/physiology Exhalation/physiology Humans Male Mass Spectrometry/*methods Mentha piperita Models, Theoretical Plant Oils/chemistry Reproducibility of Results Volatile Organic Compounds/analysis Young Adult;" |
| Notes: | "MedlineRosenthal, Kerry Ruszkiewicz, Dorota M Allen, Hayden Lindley, Martin R Turner, Matthew A Hunsicker, Eugenie eng Clinical Trial England 2019/07/26 J Breath Res. 2019 Sep 30; 13(4):046013. doi: 10.1088/1752-7163/ab34d4" |
