| Title: | Chemical Profiling of Volatile Organic Compounds in the Headspace of Algal Cultures as Early Biomarkers of Algal Pond Crashes |
| Author(s): | Reese KL; Fisher CL; Lane PD; Jaryenneh JD; Moorman MW; Jones AD; Frank M; Lane TW; |
| Address: | "Biosciences and Biotechnology Division, Physical & Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, 94550, USA. Department of Chemistry, Michigan State University, East Lansing, MI, 48823, USA. Systems Biology Department, Sandia National Laboratories, PO Box 969, Livermore, CA, 94551, USA. Nano and Micro Sensors Department, Sandia National Laboratories, PO Box 5800, MS 0892, Albuquerque, NM, 87185, USA. Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, 48823, USA. Systems Biology Department, Sandia National Laboratories, PO Box 969, Livermore, CA, 94551, USA. twlane@sandia.gov" |
| DOI: | 10.1038/s41598-019-50125-z |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Algae ponds used in industrial biomass production are susceptible to pathogen or grazer infestation, resulting in pond crashes with high economic costs. Current methods to monitor and mitigate unhealthy ponds are hindered by a lack of early indicators that precede culture crash. We used solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS) to identify volatiles emitted from healthy and rotifer infested cultures of Microchloropsis salina. After 48 hours of algal growth, marine rotifers, Brachionus plicatilis, were added to the algae cultures and volatile organic compounds (VOC) were sampled from the headspace using SPME fibers. A GC-MS approach was used in an untargeted analysis of VOCs, followed by preliminary identification. The addition of B. plicatilis to healthy cultures of M. salina resulted in decreased algal cell numbers, relative to uninfected controls, and generated trans-beta-ionone and beta-cyclocitral, which were attributed to carotenoid degradation. The abundances of the carotenoid-derived VOCs increased with rotifer consumption of algae. Our results indicate that specific VOCs released by infected algae cultures may be early indicators for impending pond crashes, providing a useful tool to monitor algal biomass production and pond crash prevention" |
| Keywords: | Animals Biomarkers/analysis Ecology Environmental Biomarkers *Eutrophication Ponds/*chemistry/microbiology Rotifera Volatile Organic Compounds/*analysis/metabolism; |
| Notes: | "MedlineReese, Kristen L Fisher, Carolyn L Lane, Pamela D Jaryenneh, James D Moorman, Matthew W Jones, A Daniel Frank, Matthias Lane, Todd W eng Research Support, U.S. Gov't, Non-P.H.S. England 2019/09/27 Sci Rep. 2019 Sep 25; 9(1):13866. doi: 10.1038/s41598-019-50125-z" |
