| Title: | Cross-validation of the peppermint benchmarking experiment across three analytical platforms |
| Author(s): | Pham YL; Yu R; Beauchamp J; |
| Address: | "Fraunhofer Institute for Process Engineering and Packaging IVV, Freising, Germany. Chair of Aroma and Smell Research, Department of Chemistry and Pharmacy, Friedrich-Alexander Universitat Erlangen-Nurnberg, Erlangen, Germany" |
| ISSN/ISBN: | 1752-7163 (Electronic) 1752-7155 (Linking) |
| Abstract: | "The Peppermint Experiment is a breath analysis benchmarking initiative that seeks to address the lack of inter-comparability of outcomes across independent breath biomarker studies. In this experiment, the washout profiles of volatile terpene constituents of encapsulated peppermint oil (mainlyalpha-pinene,beta-pinene, limonene and 1,8-cineole) in exhaled breath are characterized through a series of measurements at defined sampling intervals up to 6 h after ingestion of the capsule. In the present work, the Peppermint Experiment was carried out on a cohort of volunteers (n= 11) that provided breath samples in three sittings on different days (i.e. triplicates per volunteer) for concurrent analysis by three different analytical platforms. These platforms were proton transfer reaction-time-of-flight-mass spectrometry (PTR-TOFMS) interfaced with a buffered end-tidal (BET) breath sampler, gas chromatography-ion mobility spectrometry (GC-IMS) in conjunction with a compatible handheld direct breath sampler, and thermal desorption comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (TD-GCxGC-TOFMS) with a Respiration Collection forin-vitroAnalysis (ReCIVA) system for trapping breath volatiles onto adsorbent tubes. Regression analysis yielded mean washout times across the cohort of 448 min (PTR-TOFMS and GC-IMS) and 372 min (TD-GCxGC-TOFMS), which are in good alignment with published benchmark values. Large variations in washout profiles were observed at the individuals level, both between (inter-individual) and within (intra-individual) participants, indicating high variability in the degree of absorption, distribution, metabolism and excretion of volatile terpenes in the body within individuals and across the cohort. The comparably low inter-instrument variability indicates that differences in benchmark values from independent studies reported in the literature are driven by biological variability rather than different performances between sampling methods or analytical platforms" |
| Keywords: | Humans *Benchmarking Mentha piperita Breath Tests/methods *Volatile Organic Compounds/analysis Gas Chromatography-Mass Spectrometry/methods Protons GC x GC-MS Gc-ims Ptr-ms benchmarking peppermint pharmacokinetics standardization; |
| Notes: | "MedlinePham, Y Lan Yu, Ruyi Beauchamp, Jonathan eng Research Support, Non-U.S. Gov't England 2023/07/20 J Breath Res. 2023 Jul 31; 17(4). doi: 10.1088/1752-7163/ace8b2" |
