| Title: | Acid labile ComX pheromone from Bacillus mojavensis RO-H-1 |
| Author(s): | Okada M; Yamaguchi H; Sato I; Tsuji F; Qi J; Dubnau D; Sakagami Y; |
| Address: | "Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan. okada-org@mail.tains.tohoku.ac.jp" |
| Journal Title: | Biosci Biotechnol Biochem |
| ISSN/ISBN: | 0916-8451 (Print) 0916-8451 (Linking) |
| Abstract: | "Bacillus mojavensis strain RO-H-1 produces a posttranslationally modified hexapeptide, the ComX(RO-H-1) pheromone, that stimulates natural genetic competence controlled by quorum sensing. LC/ESI-MS analysis of partially purification of the ComX(RO-H-1) pheromone suggested a precise modification in its tryptophan residue. The corresponding ComX(RO-H-1) pheromone prepared by solid-phase synthesis was identical to the natural pheromone, and showed significant biological activity. These results indicated that the posttranslational modification of the ComX(RO-H-1) pheromone was geranylation on the tryptophan residue, resulting in the formation of a tricyclic structure. The ComX(RO-H-1) pheromone was immediately dehydrated by acid because of its extreme acid lability" |
| Keywords: | Bacillus/*metabolism Bacterial Proteins/chemistry/isolation & purification/*metabolism; |
| Notes: | "MedlineOkada, Masahiro Yamaguchi, Hisao Sato, Isao Tsuji, Fumitada Qi, Jianhua Dubnau, David Sakagami, Youji eng R01 GM057720/GM/NIGMS NIH HHS/ GM57720/GM/NIGMS NIH HHS/ Comparative Study Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England 2007/07/10 Biosci Biotechnol Biochem. 2007 Jul; 71(7):1807-10. doi: 10.1271/bbb.70245. Epub 2007 Jul 7" |
