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Biosci Biotechnol Biochem

Title:		"C-terminal identification of AD74, a proteolytic product of Enterococcus faecalis aggregation substance: application of liquid chromatography/mass spectrometry"
Author(s):		Nakayama J; Watarai H; Isogai A; Clewell DB; Suzuki A;
Address:		"Department of Agricultural Chemistry, University of Tokyo, Japan"
Journal Title:		Biosci Biotechnol Biochem
Year:		1992
Volume:		56
Issue:		1
Page Number:		127 - 131
DOI:		10.1271/bbb.56.127
ISSN/ISBN:		0916-8451 (Print) 0916-8451 (Linking)
Abstract:		"Sexual aggregation involved in conjugative transfer of Enterococcus faecalis plasmid pAD1 is enhanced by the sex pheromone cAD1, which is excreted from recipient cells. A membrane-anchored 137 kDa protein is a pAD1-encoded aggregation substance designated asal, which is responsible for cell-cell contact and leads to the aggregation of cells. An AD74 protein is a proteolytic product corresponding to the N-terminal half of asal. The C-terminal of AD74 was identified as lysine at position 510 (K-510) by liquid chromatography/mass spectrometry (LC/MS): it indicates that asal is cleaved specifically between K-510 and G-511"
Keywords:		"Amino Acid Sequence Bacterial Proteins/chemistry/genetics *Cell Adhesion Molecules Cell Aggregation Chromatography, High Pressure Liquid Enterococcus faecalis/*chemistry/genetics Mass Spectrometry Molecular Sequence Data Peptide Fragments/chemistry Peptid;"
Notes:		"MedlineNakayama, J Watarai, H Isogai, A Clewell, D B Suzuki, A eng Research Support, Non-U.S. Gov't England 1992/01/01 Biosci Biotechnol Biochem. 1992 Jan; 56(1):127-31. doi: 10.1271/bbb.56.127"