| Title: | Detection of volatile organic compounds in Brucella abortus-seropositive bison |
| Author(s): | Bayn A; Nol P; Tisch U; Rhyan J; Ellis CK; Haick H; |
| Address: | "The Department of Chemical Engineering and Russell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology , Haifa 3200003, Israel" |
| ISSN/ISBN: | 1520-6882 (Electronic) 0003-2700 (Linking) |
| Abstract: | "Brucellosis is of great public health and economic importance worldwide. Detection of brucellosis currently relies on serologic testing of an antibody response to Brucella infection, which suffers from cross-sensitivities to other antibody responses. Here we present a new method for identifying Brucella exposure that is based on profiling volatile organic compounds (VOCs) in exhaled breath. Breath samples from Brucella-seropositive bison and controls were chemically analyzed and demonstrated statistically significant differences in the concentration profiles of five VOCs. A point-of-care device incorporating an array of nanomaterial-based sensors could identify VOC patterns indicative of Brucella exposure with excellent discriminative power, using a statistical algorithm. We show that the patterns were not affected by the animals' environment and that the discriminative power of the approach was stable over time. The Brucella-indicative VOCs and collective patterns that were identified in this pilot study could lead to the development of a novel diagnostic screening test for quickly detecting infected animals chute-side, pen-side, or even remotely in populations of free-ranging ungulates. The promising preliminary results presented encourage subsequent larger scale trials in order to further evaluate the proposed method" |
| Keywords: | "Algorithms Animals Antibodies, Bacterial/immunology Biosensing Techniques Bison/*microbiology Breath Tests/*methods Brucella abortus/*pathogenicity Brucellosis/*diagnosis/transmission/veterinary Case-Control Studies Female Gas Chromatography-Mass Spectrom;" |
| Notes: | "MedlineBayn, Alona Nol, Pauline Tisch, Ulrike Rhyan, Jack Ellis, Christine K Haick, Hossam eng Comparative Study Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2013/10/26 Anal Chem. 2013 Nov 19; 85(22):11146-52. doi: 10.1021/ac403134f. Epub 2013 Nov 8" |
