| Title: | Behavioural and Electrophysiological Responses of Female Anopheles gambiae Mosquitoes to Volatiles from a Mango Bait |
| Author(s): | Meza FC; Roberts JM; Sobhy IS; Okumu FO; Tripet F; Bruce TJA; |
| Address: | "Centre for Applied Entomology and Parasitology, School of Life Sciences, Keele University, Huxley Building, Keele, Staffordshire, ST5 5BG, UK. Environmental Health and Ecological Sciences Department, Ifakara Health Institute, Of Mlabani Passage, P.O. Box 53, Ifakara, Tanzania. Centre for Integrated Pest Management, Department of Crop and Environment Sciences, Harper Adams University, Newport, Shropshire, TF10 8NB, UK. Department of Plant Protection, Faculty of Agriculture, Suez Canal university, 41522, Ismailia, Egypt. Centre for Applied Entomology and Parasitology, School of Life Sciences, Keele University, Huxley Building, Keele, Staffordshire, ST5 5BG, UK. t.j.a.bruce@keele.ac.uk" |
| DOI: | 10.1007/s10886-020-01172-8 |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Print) 0098-0331 (Linking) |
| Abstract: | "Attractive Toxic Sugar Baits (ATSB) are used in a 'lure-and-kill' approach for management of the malaria vector Anopheles gambiae, but the active chemicals were previously unknown. Here we collected volatiles from a mango, Mangifera indica, juice bait which is used in ATSBs in Tanzania and tested mosquito responses. In a Y-tube olfactometer, female mosquitoes were attracted to the mango volatiles collected 24-48 h, 48-72 h and 72-96 h after preparing the bait but volatiles collected at 96-120 h were no longer attractive. Volatile analysis revealed emission of 23 compounds in different chemical classes including alcohols, aldehydes, alkanes, benzenoids, monoterpenes, sesquiterpenes and oxygenated terpenes. Coupled GC-electroantennogram (GC-EAG) recordings from the antennae of An. gambiae showed robust responses to 4 compounds: humulene, (E)-caryophyllene, terpinolene and myrcene. In olfactometer bioassays, mosquitoes were attracted to humulene and terpinolene. (E)-caryophyllene was marginally attractive while myrcene elicited an avoidance response with female mosquitoes. A blend of humulene, (E)-caryophyllene and terpinolene was highly attractive to females (P < 0.001) when tested against a solvent blank. Furthermore, there was no preference when this synthetic blend was offered as a choice against the natural sample. Our study has identified the key compounds from mango juice baits that attract An. gambiae and this information may help to improve the ATSBs currently used against malaria vectors" |
| Keywords: | Animals Anopheles/*physiology Chemotaxis Feeding Behavior Female Food Chain *Insect Control Mangifera/*chemistry Pheromones/*metabolism Volatile Organic Compounds/*metabolism Attractant Kairomone Malaria vector Mango Terpenoids; |
| Notes: | "MedlineMeza, Felician C Roberts, Joe M Sobhy, Islam S Okumu, Fredros O Tripet, Frederic Bruce, Toby J A eng WT_/Wellcome Trust/United Kingdom 208322/Z/17/Z/WT_/Wellcome Trust/United Kingdom 2020/04/11 J Chem Ecol. 2020 Apr; 46(4):387-396. doi: 10.1007/s10886-020-01172-8. Epub 2020 Apr 9" |
