| Title: | Chitosan Modulates Volatile Organic Compound Emission from the Biocontrol Fungus Pochonia chlamydosporia |
| Author(s): | Mestre-Tomas J; Esgueva-Vila D; Fuster-Alonso A; Lopez-Moya F; Lopez-Llorca LV; |
| Address: | "Laboratory of Plant Pathology, Department of Marine Sciences and Applied Biology, University of Alicante, 03690 Alicante, Spain. Institute for Integrative Systems Biology (CSIC-UV), Spanish National Research Council, 46980 Paterna, Spain. Institut de Ciencies del Mar (ICM-CSIC), Renewable Marine Resources Department, 08003 Barcelona, Spain" |
| DOI: | 10.3390/molecules28104053 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Fungal volatile organic compounds (VOCs) are responsible for fungal odor and play a key role in biological processes and ecological interactions. VOCs represent a promising area of research to find natural metabolites for human exploitation. Pochonia chlamydosporia is a chitosan-resistant nematophagous fungus used in agriculture to control plant pathogens and widely studied in combination with chitosan. The effect of chitosan on the production of VOCs from P. chlamydosporia was analyzed using gas chromatography-mass spectrometry (GC-MS). Several growth stages in rice culture medium and different times of exposure to chitosan in modified Czapek-Dox broth cultures were analyzed. GC-MS analysis resulted in the tentative identification of 25 VOCs in the rice experiment and 19 VOCs in the Czapek-Dox broth cultures. The presence of chitosan in at least one of the experimental conditions resulted in the de novo production of 3-methylbutanoic acid and methyl 2,4-dimethylhexanoate, and oct-1-en-3-ol and tetradec-1-ene in the rice and Czapek-Dox experiments, respectively. Other VOCs changed their abundance because of the effect of chitosan and fungal age. Our findings suggest that chitosan can be used as a modulator of the production of VOCs in P. chlamydosporia and that there is also an effect of fungal age and exposure time" |
| Keywords: | Humans *Chitosan/pharmacology *Volatile Organic Compounds/pharmacology *Hypocreales/metabolism Pochonia chalmydosporia chitosan gas chromatography mass spectrometry volatile organic compounds; |
| Notes: | "MedlineMestre-Tomas, Jorge Esgueva-Vila, David Fuster-Alonso, Alba Lopez-Moya, Federico Lopez-Llorca, Luis V eng PID2020-119734RB-I00 Project/Spanish Ministry of Science and Innovation/ 727624./European Project H2020 MUSA/ Switzerland 2023/05/27 Molecules. 2023 May 12; 28(10):4053. doi: 10.3390/molecules28104053" |
