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Anal Bioanal Chem

Title:		Monoterpene separation by coupling proton transfer reaction time-of-flight mass spectrometry with fastGC
Author(s):		Materic D; Lanza M; Sulzer P; Herbig J; Bruhn D; Turner C; Mason N; Gauci V;
Address:		"Department of Environment, Earth and Ecosystems, The Open University, Walton Hall, Milton Keynes, MK7 6AA, UK. dusan.materic@open.ac.uk. IONICON Analytik, Eduard-Bodem-Gasse 3, 6020, Innsbruck, Austria. Department of Environment, Earth and Ecosystems, The Open University, Walton Hall, Milton Keynes, MK7 6AA, UK. Department of Life, Health and Chemical Sciences, The Open University, Walton Hall, Milton Keynes, MK7 6AA, UK. Department of Physical Sciences, The Open University, Walton Hall, Milton Keynes, MK7 6AA, UK"
Journal Title:		Anal Bioanal Chem
Year:		2015
Volume:		20150808
Issue:		25
Page Number:		7757 - 7763
DOI:		10.1007/s00216-015-8942-5
ISSN/ISBN:		1618-2650 (Electronic) 1618-2642 (Linking)
Abstract:		"Proton transfer reaction mass spectrometry (PTR-MS) is a well-established technique for real-time analysis of volatile organic compounds (VOCs). Although it is extremely sensitive (with sensitivities of up to 4500 cps/ppbv, limits of detection <1 pptv and the response times of approximately 100 ms), the selectivity of PTR-MS is still somewhat limited, as isomers cannot be separated. Recently, selectivity-enhancing measures, such as manipulation of drift tube parameters (reduced electric field strength) and using primary ions other than H3O(+), such as NO(+) and O2 (+), have been introduced. However, monoterpenes, which belong to the most important plant VOCs, still cannot be distinguished so more traditional technologies, such as gas chromatography mass spectrometry (GC-MS), have to be utilised. GC-MS is very time consuming (up to 1 h) and cannot be used for real-time analysis. Here, we introduce a sensitive, near-to-real-time method for plant monoterpene research-PTR-MS coupled with fastGC. We successfully separated and identified six of the most abundant monoterpenes in plant studies (alpha- and beta-pinenes, limonene, 3-carene, camphene and myrcene) in less than 80 s, using both standards and conifer branch enclosures (Norway spruce, Scots pine and black pine). Five monoterpenes usually present in Norway spruce samples with a high abundance were separated even when the compound concentrations were diluted to 20 ppbv. Thus, fastGC-PTR-ToF-MS was shown to be an adequate one-instrument solution for plant monoterpene research"
Keywords:		Acyclic Monoterpenes Alkenes/analysis/isolation & purification Bicyclic Monoterpenes Bridged Bicyclo Compounds/analysis/isolation & purification Gas Chromatography-Mass Spectrometry/*methods Monoterpenes/*analysis/isolation & purification Picea/*chemistry;
Notes:		"MedlineMateric, Dusan Lanza, Matteo Sulzer, Philipp Herbig, Jens Bruhn, Dan Turner, Claire Mason, Nigel Gauci, Vincent eng Research Support, Non-U.S. Gov't Germany 2015/08/09 Anal Bioanal Chem. 2015 Oct; 407(25):7757-63. doi: 10.1007/s00216-015-8942-5. Epub 2015 Aug 8"