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Environ Microbiol

Title:		2-Tridecanone impacts surface-associated bacterial behaviours and hinders plant-bacteria interactions
Author(s):		Lopez-Lara IM; Nogales J; Pech-Canul A; Calatrava-Morales N; Bernabeu-Roda LM; Duran P; Cuellar V; Olivares J; Alvarez L; Palenzuela-Bretones D; Romero M; Heeb S; Camara M; Geiger O; Soto MJ;
Address:		"Centro de Ciencias Genomicas, Universidad Nacional Autonoma de Mexico, Cuernavaca, C.P. 62210, Mexico. Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas (CSIC), Granada, 18008, Spain. Centro de Investigaciones Quimicas, Universidad Autonoma del Estado de Morelos, Cuernavaca, C.P. 62209, Mexico. Centre for Biomolecular Sciences, School of Life Sciences, University of Nottingham University Park, Nottingham, NG7 2RD, UK"
Journal Title:		Environ Microbiol
Year:		2018
Volume:		20180330
Issue:		6
Page Number:		2049 - 2065
DOI:		10.1111/1462-2920.14083
ISSN/ISBN:		1462-2920 (Electronic) 1462-2912 (Linking)
Abstract:		"Surface motility and biofilm formation are behaviours which enable bacteria to infect their hosts and are controlled by different chemical signals. In the plant symbiotic alpha-proteobacterium Sinorhizobium meliloti, the lack of long-chain fatty acyl-coenzyme A synthetase activity (FadD) leads to increased surface motility, defects in biofilm development and impaired root colonization. In this study, analyses of lipid extracts and volatiles revealed that a fadD mutant accumulates 2-tridecanone (2-TDC), a methylketone (MK) known as a natural insecticide. Application of pure 2-TDC to the wild-type strain phenocopies the free-living and symbiotic behaviours of the fadD mutant. Structural features of the MK determine its ability to promote S. meliloti surface translocation, which is mainly mediated by a flagella-independent motility. Transcriptomic analyses showed that 2-TDC induces differential expression of iron uptake, redox and stress-related genes. Interestingly, this MK also influences surface motility and impairs biofilm formation in plant and animal pathogenic bacteria. Moreover, 2-TDC not only hampers alfalfa nodulation but also the development of tomato bacterial speck disease. This work assigns a new role to 2-TDC as an infochemical that affects important bacterial traits and hampers plant-bacteria interactions by interfering with microbial colonization of plant tissues"
Keywords:		"Bacterial Proteins/genetics/*metabolism Biofilms/drug effects Gene Expression Profiling Gene Expression Regulation, Bacterial Ketones/*metabolism/*pharmacology Medicago sativa/*microbiology Mutation Phenotype Sinorhizobium meliloti/*drug effects/genetics/;"
Notes:		"MedlineLopez-Lara, Isabel M Nogales, Joaquina Pech-Canul, Angel Calatrava-Morales, Nieves Bernabeu-Roda, Lydia M Duran, Paloma Cuellar, Virginia Olivares, Jose Alvarez, Laura Palenzuela-Bretones, Diana Romero, Manuel Heeb, Stephan Camara, Miguel Geiger, Otto Soto, Maria J eng BIO2013-42801-P/Spanish Ministry for Economy and Competitiveness/International ERDF funds/International 153998/CONACyT-Mexico/International MR/N501852/1/JPI-AMR-Medical Research Council/International Spanish Ministry for Education and Science/International CVI-03541/Consejeria de Economia, Innovacion Ciencia y Empleo, Junta de Andalucia/International BIO2010-18005/Spanish Ministry for Economy and Competitiveness/International MR/N501852/1/MRC_/Medical Research Council/United Kingdom Research Support, Non-U.S. Gov't England 2018/03/01 Environ Microbiol. 2018 Jun; 20(6):2049-2065. doi: 10.1111/1462-2920.14083. Epub 2018 Mar 30"