| Title: | Emission of floral volatiles is facilitated by cell-wall non-specific lipid transfer proteins |
| Author(s): | Liao P; Maoz I; Shih ML; Lee JH; Huang XQ; Morgan JA; Dudareva N; |
| Address: | "Department of Biochemistry, Purdue University, West Lafayette, IN, 47907-2063, USA. Purdue Center for Plant Biology, Purdue University, West Lafayette, IN, 47907, USA. Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China. Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, PO Box 15159, HaMaccabim Road 68, Rishon LeZion, 7505101, Israel. Davidson School of Chemical Engineering, Purdue University, West Lafayette, IN, 47907-2100, USA. Department of Biochemistry, Purdue University, West Lafayette, IN, 47907-2063, USA. dudareva@purdue.edu. Purdue Center for Plant Biology, Purdue University, West Lafayette, IN, 47907, USA. dudareva@purdue.edu. Department of Horticulture and Landscape Architecture, Purdue University, 625 Agriculture Mall Drive, West Lafayette, IN, 47907-2010, USA. dudareva@purdue.edu" |
| DOI: | 10.1038/s41467-023-36027-9 |
| ISSN/ISBN: | 2041-1723 (Electronic) 2041-1723 (Linking) |
| Abstract: | "For volatile organic compounds (VOCs) to be released from the plant cell into the atmosphere, they have to cross the plasma membrane, the cell wall, and the cuticle. However, how these hydrophobic compounds cross the hydrophilic cell wall is largely unknown. Using biochemical and reverse-genetic approaches combined with mathematical simulation, we show that cell-wall localized non-specific lipid transfer proteins (nsLTPs) facilitate VOC emission. Out of three highly expressed nsLTPs in petunia petals, which emit high levels of phenylpropanoid/benzenoid compounds, only PhnsLTP3 contributes to the VOC export across the cell wall to the cuticle. A decrease in PhnsLTP3 expression reduces volatile emission and leads to VOC redistribution with less VOCs reaching the cuticle without affecting their total pools. This intracellular build-up of VOCs lowers their biosynthesis by feedback downregulation of phenylalanine precursor supply to prevent self-intoxication. Overall, these results demonstrate that nsLTPs are intrinsic members of the VOC emission network, which facilitate VOC diffusion across the cell wall" |
| Keywords: | *Volatile Organic Compounds/metabolism Diffusion Phenylalanine Cell Wall/metabolism Lipids; |
| Notes: | "MedlineLiao, Pan Maoz, Itay Shih, Meng-Ling Lee, Ji Hee Huang, Xing-Qi Morgan, John A Dudareva, Natalia eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2023/01/20 Nat Commun. 2023 Jan 19; 14(1):330. doi: 10.1038/s41467-023-36027-9" |
