| Title: | Identification of a lipase gene with a role in tomato fruit short-chain fatty acid-derived flavor volatiles by genome-wide association |
| Author(s): | Li X; Tieman D; Liu Z; Chen K; Klee HJ; |
| Address: | "Horticultural Sciences, Genetics Institute, University of Florida, Gainesville, FL, 32611, USA. Laboratory of Fruit Quality Biology/Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Zijingang Campus, Hangzhou, 310058, PR China" |
| ISSN/ISBN: | 1365-313X (Electronic) 0960-7412 (Linking) |
| Abstract: | "Fatty acid-derived volatile organic compounds (FA-VOCs) make significant contributions to tomato (Solanum lycopersicum) fruit flavor and human preferences. Short-chain FA-VOCs (C5 and C6) are among the most abundant and important volatile compounds in tomato fruits. The precursors of these volatiles, linoleic acid (18:2) and linolenic acid (18:3), are derived from cleavage of glycerolipids. However, the initial step in synthesis of these FA-VOCs has not been established. A metabolite-based genome-wide association study combined with genetic mapping and functional analysis identified a gene encoding a novel class III lipase family member, Sl-LIP8, that is associated with accumulation of short-chain FA-VOCs in tomato fruit. In vitro assays indicated that Sl-LIP8 can cleave 18:2 and 18:3 acyl groups from glycerolipids. A CRISPR/Cas9 gene edited Sl-LIP8 mutant had much lower content of multiple fruit short-chain FA-VOCs, validating an important role for this enzyme in the pathway. Sl-LIP8 RNA abundance was correlated with FA-VOC content, consistent with transcriptional regulation of the first step in the pathway. Taken together, our work indicates that glycerolipid turnover by Sl-LIP8 is an important early step in the synthesis of multiple short-chain FA-VOCs" |
| Keywords: | Fatty Acids/metabolism Fruit/genetics/*metabolism Genome-Wide Association Study Hexanols/metabolism Lipase/genetics/*metabolism Solanum lycopersicum/genetics/*metabolism Solanum lycopersicum FA-VOCs Z-3-hexen-1-ol fatty acids glycerolipids hexyl alcohol l; |
| Notes: | "MedlineLi, Xiang Tieman, Denise Liu, Zimeng Chen, Kunsong Klee, Harry J eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2020/08/14 Plant J. 2020 Nov; 104(3):631-644. doi: 10.1111/tpj.14951. Epub 2020 Sep 3" |
