| Title: | Disruption of a silencer domain by a retrotransposon |
| Author(s): | Mastrangelo MF; Weinstock KG; Shafer BK; Hedge AM; Garfinkel DJ; Strathern JN; |
| Address: | "Laboratory of Eukaryotic Gene Expression, NCI-Frederick Cancer Research and Development Center, Maryland 21702-1201" |
| DOI: | 10.1093/genetics/131.3.519 |
| ISSN/ISBN: | 0016-6731 (Print) 0016-6731 (Linking) |
| Abstract: | "A galactose-inducible Ty element carrying the HIS3 gene has been used as an insertional mutagen to generate alpha-factor resistant mutants. This collection of Ty-induced mutations includes insertions into the gene for the alpha-factor receptor (STE2), several nonspecific STE genes, and mutations that lead to the expression of the normally silent HML alpha locus. The hml alpha 'on' mutations fall into two classes, those that disrupt trans-acting regulators involved in silencing HML alpha and a novel class of mutations that activate HML alpha by insertion at that locus. The hml alpha::Ty 'on' mutations illustrate the unusual ability of these retrotransposons to activate genes by overcoming gene silencing mechanisms. The hml alpha::Ty 'on' mutations include examples of multimeric Ty arrays. Single Ty and solo delta insertion derivatives of these Ty multimers restore the ability of the silencing mechanism to repress HML alpha" |
| Keywords: | "Blotting, Southern *DNA Transposable Elements Electrophoresis, Gel, Pulsed-Field *Gene Expression Regulation, Fungal Genes, Fungal Mating Factor Mutagenesis, Insertional Peptides/*genetics Phenotype Receptors, Cell Surface/genetics Receptors, Mating Facto;" |
| Notes: | "MedlineMastrangelo, M F Weinstock, K G Shafer, B K Hedge, A M Garfinkel, D J Strathern, J N eng N01-CO-74101/CO/NCI NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 1992/07/01 Genetics. 1992 Jul; 131(3):519-29. doi: 10.1093/genetics/131.3.519" |
