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Nano Lett

Title:		Cellogram: On-the-Fly Traction Force Microscopy
Author(s):		Lendenmann T; Schneider T; Dumas J; Tarini M; Giampietro C; Bajpai A; Chen W; Gerber J; Poulikakos D; Ferrari A; Panozzo D;
Address:		"Laboratory of Thermodynamics in Emerging Technologies and #Institute for Mechanical Systems , ETH Zurich , Zurich 8092 , Switzerland. Courant Institute of Mathematical Sciences , New York University , New York 10003 , United States. nTopology , New York 10013 , United States. Department of Computer Science , Universita degli Studi di Milano , Milano 20133 , Italy. Swiss Federal Laboratories for Materials Science and Technology , EMPA , Dubendorf 8600 , Switzerland. Department of Mechanical and Aerospace Engineering , New York University , New York 11201 , United States"
Journal Title:		Nano Lett
Year:		2019
Volume:		20190925
Issue:		10
Page Number:		6742 - 6750
DOI:		10.1021/acs.nanolett.9b01505
ISSN/ISBN:		1530-6992 (Electronic) 1530-6984 (Linking)
Abstract:		"Traction force microscopy (TFM) derives maps of cell-generated forces, typically in the nanonewton range, transmitted to the extracellular environment upon actuation of complex biological processes. In traditional approaches, force rendering requires a terminal, time-consuming step of cell deadhesion to obtain a reference image. A conceptually opposite approach is provided by reference-free methods, opening to the on-the-fly generation of force maps from an ongoing experiment. This requires an image processing algorithm keeping the pace of the biological phenomena under investigation. Here, we introduce an integrated software pipeline rendering force maps from single reference-free TFM images seconds to minutes after their acquisition. The algorithm tackles image processing, reference image estimation, and finite element analysis as a single problem, yielding a robust and fully automatic solution. The method's capabilities are demonstrated in two applications. First, the mechanical annihilation of cancer cells is monitored as a function of rising environmental temperature, setting a population threshold at 45 degrees C. Second, the fast temporal correlation of forces produced across individual cells is used to map physically connected adhesion points, yielding typical lengths that vary as a function of the cell cycle phase"
Keywords:		Real time analysis cTFM focal adhesion reference free stress fibers traction force microscopy;
Notes:		"PubMed-not-MEDLINELendenmann, Tobias Schneider, Teseo Dumas, Jeremie Tarini, Marco Giampietro, Costanza Bajpai, Apratim Chen, Weiqiang Gerber, Julia Poulikakos, Dimos Ferrari, Aldo Panozzo, Daniele eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2019/09/21 Nano Lett. 2019 Oct 9; 19(10):6742-6750. doi: 10.1021/acs.nanolett.9b01505. Epub 2019 Sep 25"