| Title: | Evidence for early intracellular accumulation of volatile compounds during spadix development in Arum italicum L. and preliminary data on some tropical Aroids |
| Author(s): | Leguet A; Gibernau M; Shintu L; Caldarelli S; Moja S; Baudino S; Caissard JC; |
| Address: | "Laboratoire BVpam, EA 3061, Universite Jean Monnet, Universite de Lyon, Saint-Etienne, France" |
| DOI: | 10.1007/s00114-014-1197-8 |
| ISSN/ISBN: | 1432-1904 (Electronic) 0028-1042 (Linking) |
| Abstract: | "Staining and histochemistry of volatile organic compounds (VOCs) were performed at different inflorescence developmental stages on nine aroid species; one temperate, Arum italicum and eight tropical from the genera Caladium, Dieffenbachia and Philodendron. Moreover, a qualitative and quantitative analysis of VOCs constituting the scent of A. italicum, depending on the stage of development of inflorescences was also conducted. In all nine species, vesicles were observed in the conical cells of either the appendix or the stamens (thecae) and the staminodes. VOCs were localised in intracellular vesicles from the early stages of inflorescence development until their release during receptivity of gynoecium. This localisation was observed by the increase of both number and diameter of the vesicles during 1 week before receptivity. Afterwards, vesicles were fewer and smaller but rarely absent. In A. italicum, staining and gas chromatography analyses confirmed that the vesicles contained terpenes. The quantitatively most important ones were the sesquiterpenes, but monoterpenes were not negligible. Indeed, the quantities of terpenes matched the vesicles' size evolution during 1 week. Furthermore, VOCs from different biosynthetic pathways (sesquiterpenes and alkanes) were at their maximum quantity 2 days before gynoecium receptivity (sesquiterpenes and alkanes) or during receptivity (isobutylamine, monoterpenes, skatole and p-cresol). VOCs seemed to be emitted during gynoecium receptivity and/or during thermogenesis, and FADs are accumulated after thermogenesis in the spadix. These complex dynamics of the different VOCs could indicate specialisation of some VOCs and cell machinery to attract pollinators on the one hand and to repulse/protect against phytophagous organisms and pathogens after pollination on the other hand" |
| Keywords: | "Araceae/*chemistry/growth & development Arum/*chemistry/growth & development Chromatography, Gas Plant Leaves/chemistry *Pollination Terpenes/analysis Volatile Organic Compounds/*analysis;" |
| Notes: | "MedlineLeguet, Aurelia Gibernau, Marc Shintu, Laetitia Caldarelli, Stefano Moja, Sandrine Baudino, Sylvie Caissard, Jean-Claude eng Research Support, Non-U.S. Gov't Germany 2014/06/14 Naturwissenschaften. 2014 Aug; 101(8):623-35. doi: 10.1007/s00114-014-1197-8. Epub 2014 Jun 13" |
