| Title: | Downy mildew symptoms on grapevines can be reduced by volatile organic compounds of resistant genotypes |
| Author(s): | Lazazzara V; Bueschl C; Parich A; Pertot I; Schuhmacher R; Perazzolli M; |
| Address: | "Department of Sustainable Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010, San Michele all'Adige, Italy. Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad-Lorenz-Strasse 20, 3430, Tulln, Austria. Centre Agriculture Food Environment, University of Trento, Via E. Mach 1, 38010, San Michele all'Adige, Italy. Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad-Lorenz-Strasse 20, 3430, Tulln, Austria. rainer.schuhmacher@boku.ac.at. Department of Sustainable Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010, San Michele all'Adige, Italy. michele.perazzolli@fmach.it" |
| DOI: | 10.1038/s41598-018-19776-2 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Volatile organic compounds (VOCs) play a crucial role in the communication of plants with other organisms and are possible mediators of plant defence against phytopathogens. Although the role of non-volatile secondary metabolites has been largely characterised in resistant genotypes, the contribution of VOCs to grapevine defence mechanisms against downy mildew (caused by Plasmopara viticola) has not yet been investigated. In this study, more than 50 VOCs from grapevine leaves were annotated/identified by headspace-solid-phase microextraction gas chromatography-mass spectrometry analysis. Following P. viticola inoculation, the abundance of most of these VOCs was higher in resistant (BC4, Kober 5BB, SO4 and Solaris) than in susceptible (Pinot noir) genotypes. The post-inoculation mechanism included the accumulation of 2-ethylfuran, 2-phenylethanol, beta-caryophyllene, beta-cyclocitral, beta-selinene and trans-2-pentenal, which all demonstrated inhibitory activities against downy mildew infections in water suspensions. Moreover, the development of downy mildew symptoms was reduced on leaf disks of susceptible grapevines exposed to air treated with 2-ethylfuran, 2-phenylethanol, beta-cyclocitral or trans-2-pentenal, indicating the efficacy of these VOCs against P. viticola in receiver plant tissues. Our data suggest that VOCs contribute to the defence mechanisms of resistant grapevines and that they may inhibit the development of downy mildew symptoms on both emitting and receiving tissues" |
| Keywords: | Anti-Infective Agents/*analysis Gas Chromatography-Mass Spectrometry Oomycetes/drug effects/*growth & development Phytochemicals/*analysis Plant Diseases/*immunology/microbiology Vitis/*chemistry/immunology/microbiology Volatile Organic Compounds/*analysi; |
| Notes: | "MedlineLazazzara, Valentina Bueschl, Christoph Parich, Alexandra Pertot, Ilaria Schuhmacher, Rainer Perazzolli, Michele eng Research Support, Non-U.S. Gov't England 2018/01/28 Sci Rep. 2018 Jan 26; 8(1):1618. doi: 10.1038/s41598-018-19776-2" |
