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J Chem Ecol

Title:		Maize genes induced by herbivory and volicitin
Author(s):		Lawrence SD; Novak NG;
Address:		"Insect Biocontrol Laboratory, USDA-ARS, BARC-West, Beltsville, MD, USA. Lawrencs@ba.ars.usda.gov"
Journal Title:		J Chem Ecol
Year:		2004
Volume:		30
Issue:		12
Page Number:		2543 - 2557
DOI:		10.1007/s10886-004-7949-8
ISSN/ISBN:		0098-0331 (Print) 0098-0331 (Linking)
Abstract:		"In crop plants, both mechanical damage and insect attack trigger rapid changes in gene transcription. We investigated whether insect herbivory differs from a general wound response, and if so, is the induction specific to the pest/host plant interaction? Herbivory by beet armyworm (BAW; Spodoptera exigua) caterpillars on maize results in a unique pattern of volatile compounds not triggered by wounding alone that attracts the generalist parasitoid Cotesia marginiventris. Caterpillar-induced volatile emission can be mimicked when a component of the BAW oral secretions (N-(17-hydroxylinolenoyl)-L-glutamine) termed volicitin, is applied to wounded leaves. We identified genes that are affected by BAW feeding by comparing volicitin treatment with wounding alone. We compared cDNAs from these two populations by isolating genes from a subtractive library and using reverse northerns. Virtual northern blots confirmed these results and further showed that BAW infestation affected the expression of these genes. In some cases, BAW feeding inhibited the expression of volicitin-induced genes, suggesting the role of additional bioactive components in caterpillar regurgitate. Transcripts involved in volatile production are increased by volicitin and BAW infestation treatments, and are also detectable at low levels in mechanically wounded leaves. Finally, we identified three new sesquiterpene cyclase genes that are induced by volicitin"
Keywords:		"Animals Base Sequence Carbon-Carbon Lyases/genetics/metabolism DNA, Complementary/drug effects/genetics Gene Expression Regulation/*drug effects *Genes, Plant Glutamine/*analogs & derivatives/*pharmacology Host-Parasite Interactions/*genetics Plant Diseas;"
Notes:		"MedlineLawrence, Susan D Novak, Nicole G eng Comparative Study 2005/02/24 J Chem Ecol. 2004 Dec; 30(12):2543-57. doi: 10.1007/s10886-004-7949-8"