| Title: | Differences in the Emission of Volatile Organic Compounds (VOCs) between Non-Differentiating and Adipogenically Differentiating Mesenchymal Stromal/Stem Cells from Human Adipose Tissue |
| Author(s): | Klemenz AC; Meyer J; Ekat K; Bartels J; Traxler S; Schubert JK; Kamp G; Miekisch W; Peters K; |
| Address: | "Department of Anesthesiology and Intensive Care Medicine, University Medical Centre Rostock, Schillingallee 35, 18057 Rostock, Germany. Department of Cell Biology, University Medical Centre Rostock, Schillingallee 69, 18057 Rostock, Germany. AMP-Lab GmbH, Mendelstr. 11, 48149 Munster, Germany. Department of Cell Biology, University Medical Centre Rostock, Schillingallee 69, 18057 Rostock, Germany. kirsten.peters@med.uni-rostock.de" |
| ISSN/ISBN: | 2073-4409 (Print) 2073-4409 (Electronic) 2073-4409 (Linking) |
| Abstract: | "Metabolic characterization of human adipose tissue-derived mesenchymal stromal/stem cells (ASCs) is of importance in stem cell research. The monitoring of the cell status often requires cell destruction. An analysis of volatile organic compounds (VOCs) in the headspace above cell cultures might be a noninvasive and nondestructive alternative to in vitro analysis. Furthermore, VOC analyses permit new insight into cellular metabolism due to their view on volatile compounds. Therefore, the aim of our study was to compare VOC profiles in the headspace above nondifferentiating and adipogenically differentiating ASCs. To this end, ASCs were cultivated under nondifferentiating and adipogenically differentiating conditions for up to 21 days. At different time points the headspace samples were preconcentrated by needle trap micro extraction and analyzed by gas chromatography/mass spectrometry. Adipogenic differentiation was assessed at equivalent time points. Altogether the emissions of 11 VOCs showed relevant changes and were analyzed in more detail. A few of these VOCs, among them acetaldehyde, were significantly different in the headspace of adipogenically differentiating ASCs and appeared to be linked to metabolic processes. Furthermore, our data indicate that VOC headspace analysis might be a suitable, noninvasive tool for the metabolic monitoring of (mesenchymal stem) cells in vitro" |
| Keywords: | Adipose Tissue/*chemistry/metabolism Cell Culture Techniques Cell Differentiation Gas Chromatography-Mass Spectrometry/methods Humans Mesenchymal Stem Cells/*chemistry Volatile Organic Compounds/analysis/*chemistry adipose tissue-derived mesenchymal strom; |
| Notes: | "MedlineKlemenz, Ann-Christin Meyer, Juliane Ekat, Katharina Bartels, Julia Traxler, Selina Schubert, Jochen K Kamp, Gunter Miekisch, Wolfram Peters, Kirsten eng Research Support, Non-U.S. Gov't Switzerland 2019/07/13 Cells. 2019 Jul 10; 8(7):697. doi: 10.3390/cells8070697" |
