| Title: | Global Plasma Profiling for Colorectal Cancer-Associated Volatile Organic Compounds: a Proof-of-Principle Study |
| Author(s): | Kim S; Yin X; Prodhan MAI; Zhang X; Zhong Z; Kato I; |
| Address: | "Department of Oncology, Wayne State University School of Medicine, Detroit MI, USA. Biostatistics Core, Karmanos Cancer Institute, Wayne State University, Detroit MI, USA. Department of Chemistry, University of Louisville, Louisville, Kentucky, USA. Department of Computer Science, College of Engineering, Wayne State University, Detroit MI, USA. Department of Pathology, Wayne State University School of Medicine, Detroit MI, USA" |
| ISSN/ISBN: | 1945-239X (Electronic) 0021-9665 (Print) 0021-9665 (Linking) |
| Abstract: | "Volatile organic compounds (VOCs) could reflect changes resulting from ongoing pathophysiological processes and altered body metabolisms, and thus have been studied for various types of cancers. We aimed to test an advanced global metabolomic technique to characterize circulating VOCs in patients diagnosed with colorectal cancer (CRC). We employed solid-phase microextraction (SPME) and comprehensive two-dimensional gas chromatography mass-spectrometry (GC x GC-MS). We analyzed 30 random plasma samples from incident cases of CRC. The 30 samples were from population controls enrolled in a large population-based case-control study. The number of metabolite peaks detected in the cases was significantly lower than that detected in the controls (median 1530 vs. 1694, P = 0.02). Partial least squares-discriminant analysis showed clear VOC profile differences between the CRC and the controls. After adjustment for multiple comparisons at the 5% false discovery rate level, five VOCs were differentially expressed between the cases and the controls. Among these five VOCs, 2,3,4-trimethyl-hexane (decreased) and 2,4-dimethylhept-1-ene (increased) were both lipid peroxidation products but not previously reported for CRC. In summary, this study pointed to an intriguing observation that the richness of volatile metabolites may be reduced in CRC cases and demonstrated the utility of SPME GC x GC-MS in discovery of candidate markers for further validation" |
| Keywords: | Aged Biomarkers/blood/chemistry Case-Control Studies Colorectal Neoplasms/*blood Discriminant Analysis Female Gas Chromatography-Mass Spectrometry/*methods Humans Male Metabolomics/methods Middle Aged Plasma/*chemistry Solid Phase Microextraction Volatile; |
| Notes: | "MedlineKim, Seongho Yin, Xinmin Prodhan, Md Aminul Islam Zhang, Xiang Zhong, Zichun Kato, Ikuko eng P20 GM113226/GM/NIGMS NIH HHS/ P50 AA024337/AA/NIAAA NIH HHS/ Evaluation Study 2019/02/24 J Chromatogr Sci. 2019 May 1; 57(5):385-396. doi: 10.1093/chromsci/bmz011" |
