| Title: | [Metabolic pathways responsible for consumption of aromatic hydrocarbons by microbial associations: molecular-genetic characterization] |
| Author(s): | Khomenkov VG; Shevelev AB; Zhukov VG; Kurlovich AE; Zagustina NA; Popov VO; |
| ISSN/ISBN: | 0555-1099 (Print) 0555-1099 (Linking) |
| Abstract: | "Genes for catechol 1,2- and 2,3-dioxygenases were cloned. These enzymes hold important positions in the ortho and meta pathways of the metabolism of aromatic carbons by microbial associations that consume the following volatile organic compounds in pilot minireactors: toluene, styrene, ethyl benzene, o-xylene, m-xylene, and naphthalene. Genes of both pathways were found in an association consuming m-xylene; only genes of the ortho pathway were found in associations consuming o-xylene, styrene, and ethyl benzene, and only genes of the meta pathway were found in associations consuming naphthalene and toluene. Genes of the ortho pathway (C120) cloned from associations consuming o-xylene and ethyl benzene were similar to corresponding genes located on the pND6 plasmid of Pseudomonas putida. Genes of the ortho pathway from associations consuming o-xylene and m-xylene were similar to chromosomal genes of P. putida. Genes of the meta pathway (C230) from associations consuming toluene and naphthalene were similar to corresponding genes formerly found in plasmids pWWO and pTOL" |
| Keywords: | "Bacteria/*enzymology/genetics Catechol 1, 2-Dioxygenase Catechol 2, 3-Dioxygenase Cloning, Molecular Dioxygenases/classification/*genetics Genes, Bacterial/*genetics Hydrocarbons, Aromatic/*metabolism Phylogeny Plasmids/genetics Pseudomonas putida/enzymolog;" |
| Notes: | "MedlineKhomenkov, V G Shevelev, A B Zhukov, V G Kurlovich, A E Zagustina, N A Popov, V O rus English Abstract Russia (Federation) 2005/06/28 Prikl Biokhim Mikrobiol. 2005 May-Jun; 41(3):298-302" |
