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Genetics

Title:		The filamentous growth MAPK Pathway Responds to Glucose Starvation Through the Mig1/2 transcriptional repressors in Saccharomyces cerevisiae
Author(s):		Karunanithi S; Cullen PJ;
Address:		"Department of Biological Sciences, State University of New York, Buffalo, New York 14260-1300, USA"
Journal Title:		Genetics
Year:		2012
Volume:		20120817
Issue:		3
Page Number:		869 - 887
DOI:		10.1534/genetics.112.142661
ISSN/ISBN:		1943-2631 (Electronic) 0016-6731 (Print) 0016-6731 (Linking)
Abstract:		"In the budding yeast S. cerevisiae, nutrient limitation induces a MAPK pathway that regulates filamentous growth and biofilm/mat formation. How nutrient levels feed into the regulation of the filamentous growth pathway is not entirely clear. We characterized a newly identified MAPK regulatory protein of the filamentous growth pathway, Opy2. A two-hybrid screen with the cytosolic domain of Opy2 uncovered new interacting partners including a transcriptional repressor that functions in the AMPK pathway, Mig1, and its close functional homolog, Mig2. Mig1 and Mig2 coregulated the filamentous growth pathway in response to glucose limitation, as did the AMP kinase Snf1. In addition to associating with Opy2, Mig1 and Mig2 interacted with other regulators of the filamentous growth pathway including the cytosolic domain of the signaling mucin Msb2, the MAP kinase kinase Ste7, and the MAP kinase Kss1. As for Opy2, Mig1 overproduction dampened the pheromone response pathway, which implicates Mig1 and Opy2 as potential regulators of pathway specificity. Taken together, our findings provide the first regulatory link in yeast between components of the AMPK pathway and a MAPK pathway that controls cellular differentiation"
Keywords:		Cell Membrane/metabolism Glucose/*metabolism *MAP Kinase Signaling System Membrane Proteins/metabolism Protein Binding Protein Serine-Threonine Kinases/metabolism Repressor Proteins/*metabolism Saccharomyces cerevisiae/*growth & development/*metabolism Sa;
Notes:		"MedlineKarunanithi, Sheelarani Cullen, Paul J eng R01 GM098629/GM/NIGMS NIH HHS/ 2012/08/21 Genetics. 2012 Nov; 192(3):869-87. doi: 10.1534/genetics.112.142661. Epub 2012 Aug 17"