« Previous AbstractFaecal chemical cues in water buffalo that facilitate estrus detection    Next Abstract"Identification of Esters as Novel Aggregation Pheromone Components Produced by the Male Powder-Post Beetle, Lyctus africanus Lesne (Coleoptera: Lyctinae)" »

Zoolog Sci

Title:		Identification of p-cresol as an estrus-specific volatile in buffalo saliva: comparative docking analysis of buffalo OBP and beta-lactoglobulin with p-cresol
Author(s):		Karthikeyan K; Manivannan P; Rajesh D; Muthukumar S; Muralitharan G; Akbarsha MA; Archunan G;
Address:		"1 Center for Pheromone Technology, Department of Animal Science, Bharathidasan University, Tiruchirappalli-620024, Tamilnadu, India"
Journal Title:		Zoolog Sci
Year:		2014
Volume:		31
Issue:		1
Page Number:		31 - 36
DOI:		10.2108/zsj.31.31
ISSN/ISBN:		0289-0003 (Print) 0289-0003 (Linking)
Abstract:		"Assessment of salivary volatile compounds adopting gas chromatography-linked mass spectrometry (GC-MS) analysis revealed the presence of a total of 11 compounds in the buffalo saliva irrespective of the stages in the reproductive cycle. p-cresol was identified as an estrus-specific volatile compound in the saliva. In addition, modeling of odorant-binding protein (OBP) and beta-lactoglobulin revealed that OBP is highly stable and has strong binding affinity with p-cresol. Hydrogen bond interactions indicated that OBP is responsible for pheromone release through saliva. In contrast, beta-lactoglobulin, which belongs to the same lipocalin family as OBP, possesses less affinity to p-cresol than OBP, suggesting that it is not involved in p-cresol binding and transport. Phylogenetic characterization revealed that bovine family of OBP is separately clustered. It is suggested that p-cresol has the potential to be developed as a biomarker to detect the reproductive status in the buffalo and for behavioral manipulations"
Keywords:		"Animals Buffaloes Cresols/chemistry/*metabolism Estrus/*physiology Female Gene Expression Regulation/physiology Lactoglobulins/chemistry/*metabolism Phylogeny Protein Binding Receptors, Odorant/chemistry/*metabolism Saliva/*chemistry;"
Notes:		"MedlineKarthikeyan, Kandasamy Manivannan, Paramasivan Rajesh, Durairaj Muthukumar, Subramanian Muralitharan, Gangatharan Akbarsha, Mohammad Abdulkader Archunan, Govindaraju eng Research Support, Non-U.S. Gov't Japan 2014/01/15 Zoolog Sci. 2014 Jan; 31(1):31-6. doi: 10.2108/zsj.31.31"