« Previous AbstractCharacterized constituents of insect herbivore oral secretions and their influence on the regulation of plant defenses    Next AbstractExploring the volatile metabolome of conventional and organic walnut oils by solid-phase microextraction and analysis by GC-MS combined with chemometrics »

J Breath Res

Title:		Cell culture metabolomics in the diagnosis of lung cancer-the influence of cell culture conditions
Author(s):		Kalluri U; Naiker M; Myers MA;
Address:		"Biomedical Science, School of Health Sciences, Federation University Australia, Mt Helen, Victoria 3350, Australia"
Journal Title:		J Breath Res
Year:		2014
Volume:		20140527
Issue:		2
Page Number:		27109 -
DOI:		10.1088/1752-7155/8/2/027109
ISSN/ISBN:		1752-7163 (Electronic) 1752-7155 (Linking)
Abstract:		"Lung cancer is the leading cause of cancer deaths. Unfortunately, lung cancer is often diagnosed only when it becomes symptomatic or at an advanced stage when few treatment options are available. Hence, a diagnostic test suitable for screening widespread populations is required to enable earlier diagnosis. Analysis of exhaled breath provides a non-invasive method for early detection of lung cancer. Analysis of volatile organic compounds (VOCs) by various mass spectral techniques has identified potential biomarkers of disease. Nevertheless, the metabolic origins and the disease specificity of VOCs need further elucidation. Cell culture metabolomics can be used as a bottom-up approach to identify biomarkers of pathological conditions and can also be used to study the metabolic pathways that produce such compounds. This paper summarizes the current knowledge of lung cancer biomarkers in exhaled breath and emphasizes the critical role of cell culture conditions in determining the VOCs produced in vitro. Hypoxic culture conditions more closely mimic the conditions of cancer cell growth in vivo. We propose that since hypoxia influences cell metabolism and so potentially the VOCs that the cancer cells produce, the cell culture metabolomics projects should consider culturing cancer cells in hypoxic conditions"
Keywords:		Biomarkers/analysis/metabolism Breath Tests *Cell Culture Techniques/methods Cell Hypoxia/physiology Humans Lung Neoplasms/*diagnosis/*metabolism/pathology Metabolomics/*methods Volatile Organic Compounds/analysis/metabolism;
Notes:		"MedlineKalluri, U Naiker, M Myers, M A eng Research Support, Non-U.S. Gov't Review England 2014/05/28 J Breath Res. 2014 Jun; 8(2):027109. doi: 10.1088/1752-7155/8/2/027109. Epub 2014 May 27"