| Title: | "The DEAD-box RNA helicase, Dhh1, functions in mating by regulating Ste12 translation in Saccharomyces cerevisiae" |
| Address: | "Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University, Yuseong-Gu, Gung-Dong 220, Daejeon 305-764, Republic of Korea" |
| Journal Title: | Biochem Biophys Res Commun |
| DOI: | 10.1016/j.bbrc.2007.12.169 |
| ISSN/ISBN: | 1090-2104 (Electronic) 0006-291X (Linking) |
| Abstract: | "The DEAD-box RNA helicase, Dhh1, is a member of a highly conserved subfamily designated RCK/p54 helicases. Dhh1 functions as mRNA decapping activator, and is localized to discrete cytoplasmic foci known as processing bodies (P-bodies). Here, we describe the essential roles of Dhh1 in the yeast mating pathway. A dhh1 deletion mutation caused a significant decrease in the protein level of Ste12, a mating-specific transcription factor, resulting in severe mating defects. We examined the accumulation of Dhh1-GFP in P-bodies during mating. Following pheromone treatment, the P-body intensity and number increased in wild-type cells, while dhh1 mutant cells failed to show P-body formation. Both the mating and P-body phenotypes of dhh1 were suppressed by overexpression of STE12 or CAF20 encoding an eIF4E inhibitor. In wild-type cells, CAF20 overexpression led to an increased level of Ste12 protein as well as highly developed P-bodies. We propose that Dhh1 and Caf20 regulate the Ste12 protein expression and the Ste12 protein level is associated with P-body formation during mating" |
| Keywords: | "DEAD-box RNA Helicases/genetics/*physiology Gene Expression Regulation, Fungal/physiology Green Fluorescent Proteins/genetics Mutagenesis, Site-Directed Nuclear Cap-Binding Protein Complex/biosynthesis Organelles/metabolism Pheromones/pharmacology Protein;" |
| Notes: | "MedlineKa, Minhan Park, Young-Un Kim, Jinmi eng Research Support, Non-U.S. Gov't 2008/01/10 Biochem Biophys Res Commun. 2008 Mar 14; 367(3):680-6. doi: 10.1016/j.bbrc.2007.12.169. Epub 2008 Jan 7" |
