| Title: | Interaction between the barley allelochemical compounds gramine and hordenine and artificial lipid bilayers mimicking the plant plasma membrane |
| Author(s): | Lebecque S; Crowet JM; Lins L; Delory BM; du Jardin P; Fauconnier ML; Deleu M; |
| Address: | "TERRA-AgricultureIsLife, Gembloux Agro-Bio Tech, University of Liege, Gembloux, Belgium. Laboratory of Molecular Biophysics at Interfaces, Gembloux Agro-Bio Tech, University of Liege, Gembloux, Belgium. Ecosystem Functioning and Services, Institute of Ecology, Leuphana University, Universitatsallee 1, 21335, Luneburg, Germany. Laboratory of Plant Biology, Gembloux Agro-Bio Tech, University of Liege, Gembloux, Belgium. General and Organic Chemistry Laboratory, Gembloux Agro-Bio Tech, University of Liege, Gembloux, Belgium. marie-laure.fauconnier@ulg.ac.be. Laboratory of Molecular Biophysics at Interfaces, Gembloux Agro-Bio Tech, University of Liege, Gembloux, Belgium. magali.deleu@ulg.ac.be" |
| DOI: | 10.1038/s41598-018-28040-6 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Some plants affect the development of neighbouring plants by releasing secondary metabolites into their environment. This phenomenon is known as allelopathy and is a potential tool for weed management within the framework of sustainable agriculture. While many studies have investigated the mode of action of various allelochemicals (molecules emitted by allelopathic plants), little attention has been paid to their initial contact with the plant plasma membrane (PPM). In this paper, this key step is explored for two alkaloids, gramine and hordenine, that are allelochemicals from barley. Using in vitro bioassays, we first showed that gramine has a greater toxicity than hordenine towards a weed commonly found in northern countries (Matricaria recutita L.). Then, isothermal titration calorimetry was used to show that these alkaloids spontaneously interact with lipid bilayers that mimic the PPM. The greater impact of gramine on the thermotropic behaviour of lipids compared to hordenine was established by means of infrared spectroscopy. Finally, the molecular mechanisms of these interactions were explored with molecular dynamics simulations. The good correlation between phytotoxicity and the ability to disturb lipid bilayers is discussed. In this study, biophysical tools were used for the first time to investigate the interactions of allelochemicals with artificial PPM" |
| Keywords: | Cell Membrane/*metabolism Hordeum/*metabolism Hydrogen Bonding Indole Alkaloids/chemistry/*metabolism/toxicity Lipid Bilayers/*metabolism Liposomes Molecular Dynamics Simulation Phase Transition Pheromones/chemistry/*metabolism/toxicity Phosphatidylcholin; |
| Notes: | "MedlineLebecque, Simon Crowet, Jean-Marc Lins, Laurence Delory, Benjamin M du Jardin, Patrick Fauconnier, Marie-Laure Deleu, Magali eng PDR grant T.1003.14/Fonds De La Recherche Scientifique - FNRS (Belgian National Fund for Scientific Research)/International Grant No. 2.5020.11/Fonds De La Recherche Scientifique - FNRS (Belgian National Fund for Scientific Research)/International Research Support, Non-U.S. Gov't England 2018/06/30 Sci Rep. 2018 Jun 28; 8(1):9784. doi: 10.1038/s41598-018-28040-6" |
