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Environ Sci Pollut Res Int

Title:		"Effect of the green synthesized rGO and Mg/rGO nanocomposites on the phytochemical assay, toxicity, and metabolism of Mentha longifolia in vitro cultures"
Author(s):		Jafarirad S; Kosari-Nasab M; Aminpour M; Rezaei Z;
Address:		"Department of Organic and Biochemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran. jafarirad@tabrizu.ac.ir. Research Institute of Bioscience and Biotechnology, University of Tabriz, Tabriz, Iran. jafarirad@tabrizu.ac.ir. Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran. kosarinasabm@gmail.com. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. kosarinasabm@gmail.com. Research Institute of Bioscience and Biotechnology, University of Tabriz, Tabriz, Iran"
Journal Title:		Environ Sci Pollut Res Int
Year:		2022
Volume:		20220215
Issue:		30
Page Number:		46243 - 46258
DOI:		10.1007/s11356-022-18761-7
ISSN/ISBN:		1614-7499 (Electronic) 0944-1344 (Linking)
Abstract:		"Reduced graphene oxide (rGO) and Mg/rGO nanocomposites (NCs) were prepared by an eco-friendly technique using Rosa canina fruit extract. Physicochemical properties and cytotoxicity to Mentha longifolia in vitro cultures of these nanomaterials were examined by using XRD, FESEM, EDX, FT-IR, DLS/zeta potential, UV-Visible, and GC-MS techniques. The characterization techniques confirmed the synthesis of rGO and Mg/rGO NCs with particle sizes less than 20 nm (based on FESEM). In accordance to the biological measurements, rGO showed in vitro cytotoxicity to M. longifolia shoot cultures. Mg/rGO NCs showed no significant difference in the growth parameters except for a decrease in the shoot number at the concentrations of 50 and 150 mg/L and a decrease in the length of the tallest root at the concentrations of 100 and 150 mg/L, however efficiently improved the photosynthetic pigment contents. The phytochemical assay depicted that the total content of volatile compounds was increased in the treated cultures with 25, 50, and 100 mg/L of rGO and Mg/rGO NCs in comparison to the control. Generally, the more oxygenated and hydrocarbon sesquiterpenes were observed in the cultures treated with 25 and 100 mg/L of rGO and 25 and 50 mg/L of Mg/rGO NCs"
Keywords:		"*Graphite/chemistry/toxicity *Mentha *Nanocomposites/chemistry/toxicity Phytochemicals Spectroscopy, Fourier Transform Infrared GC-MS analysis Nanomaterials Oxygenated terpenoids Shoot cultures;"
Notes:		"MedlineJafarirad, Saeed Kosari-Nasab, Morteza Aminpour, Monireh Rezaei, Zahra eng Germany 2022/02/16 Environ Sci Pollut Res Int. 2022 Jun; 29(30):46243-46258. doi: 10.1007/s11356-022-18761-7. Epub 2022 Feb 15"