| Title: | The effects of temperature stress and population origin on the thermal sensitivity of Lymantria dispar L. (Lepidoptera: Erebidae) larvae |
| Author(s): | Ilijin L; Grcic A; Mrdakovic M; Vlahovic M; Todorovic D; Filipovic A; Matic D; Peric Mataruga V; |
| Address: | "Department of Insect Physiology and Biochemistry, Institute for Biological Research 'Sinisa Stankovic', National Institute of the Republic of Serbia, University of Belgrade, Despot Stefan Blvd. 142, 11060, Belgrade, Serbia. lararid@ibiss.bg.ac.rs. Department of Insect Physiology and Biochemistry, Institute for Biological Research 'Sinisa Stankovic', National Institute of the Republic of Serbia, University of Belgrade, Despot Stefan Blvd. 142, 11060, Belgrade, Serbia" |
| DOI: | 10.1038/s41598-022-26506-2 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Increased environmental temperature is one of the most frequent stresses effecting metabolic rate in herbivorous insect species. Our goal was to compare the influence of increased environmental temperature and induced thermotolerance on the activity of midgut phosphatases and brain tissue hsp70 concentration in 5th instar Lymantria dispar larvae originating from an unpolluted and polluted forest. Induced thermotolerance (larval pre-treatment at high, sub-lethal temperature) increases the species ability to overcome the negative effects of thermal stress, therefore we monitored the effect of this regime in larvae originating from both forests. Thermal regimes in this experiment predominantly influenced the alkaline phosphatases activity and it was affected by temperature, population origin, and their combined effect. Total acid phosphatases activity was changed only by the joint effect of temperature and population origin. Brain hsp70 concentration was under a significant individual and joint effect of temperature and population. In both populations, brain tissue hsp70 concentration and alkaline phosphatases activity should be taken under consideration as a battery with biomarker potential for thermal stress in L. dispar larvae as a bioindicator species" |
| Keywords: | Animals Larva Temperature *Moths Digestive System/metabolism HSP70 Heat-Shock Proteins/metabolism Phosphoric Monoester Hydrolases; |
| Notes: | "MedlineIlijin, Larisa Grcic, Anja Mrdakovic, Marija Vlahovic, Milena Todorovic, Dajana Filipovic, Aleksandra Matic, Dragana Peric Mataruga, Vesna eng Research Support, Non-U.S. Gov't England 2022/12/18 Sci Rep. 2022 Dec 17; 12(1):21858. doi: 10.1038/s41598-022-26506-2" |
