| Title: | New Broth Macrodilution Volatilization Method for Antibacterial Susceptibility Testing of Volatile Agents and Evaluation of Their Toxicity Using Modified MTT Assay In Vitro |
| Author(s): | Houdkova M; Chaure A; Doskocil I; Havlik J; Kokoska L; |
| Address: | "Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, 16500 Prague, Czech Republic. Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 16500 Prague, Czech Republic. Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 16500 Prague, Czech Republic" |
| DOI: | 10.3390/molecules26144179 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "In this study, a new broth macrodilution volatilization method for the simple and rapid determination of the antibacterial effect of volatile agents simultaneously in the liquid and vapor phase was designed with the aim to assess their therapeutic potential for the development of new inhalation preparations. The antibacterial activity of plant volatiles (beta-thujaplicin, thymohydroquinone, thymoquinone) was evaluated against bacteria associated with respiratory infections (Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes) and their cytotoxicity was determined using a modified thiazolyl blue tetrazolium bromide assay against normal lung fibroblasts. Thymohydroquinone and thymoquinone possessed the highest antibacterial activity against H. influenzae, with minimum inhibitory concentrations of 4 and 8 microg/mL in the liquid and vapor phases, respectively. Although all compounds exhibited cytotoxic effects on lung cells, therapeutic indices (TIs) suggested their potential use in the treatment of respiratory infections, which was especially evident for thymohydroquinone (TI > 34.13). The results demonstrate the applicability of the broth macrodilution volatilization assay, which combines the principles of broth microdilution volatilization and standard broth macrodilution methods. This assay enables rapid, simple, cost- and labor-effective screening of volatile compounds and overcomes the limitations of assays currently used for screening of antimicrobial activity in the vapor phase" |
| Keywords: | "Administration, Inhalation Anti-Bacterial Agents/analysis/*chemistry/*pharmacology Bacteria/drug effects Benzoquinones/administration & dosage/pharmacology Haemophilus influenzae/drug effects Microbial Sensitivity Tests Monoterpenes/administration & dosag;" |
| Notes: | "MedlineHoudkova, Marketa Chaure, Aishwarya Doskocil, Ivo Havlik, Jaroslav Kokoska, Ladislav eng IGA 20213109/Ceska Zemedelska Univerzita v Praze/ MEYS Grant No: LM2018100/METROFOOD-CZ research infrastructure project/ Switzerland 2021/07/25 Molecules. 2021 Jul 9; 26(14):4179. doi: 10.3390/molecules26144179" |
