| Title: | "Dynamics of plasmid-mediated niche invasion, immunity to invasion, and pheromone-inducible conjugation in the murine gastrointestinal tract" |
| Author(s): | Hirt H; Greenwood-Quaintance KE; Barnes AMT; Karau MJ; Till LM; Palzer E; Guan W; VanNieuwenhze MS; Kashyap PC; Patel R; Dunny GM; |
| Address: | "Department of Microbiology and Immunology, Medical School, University of Minnesota, Minneapolis, MN, USA. Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA. Division of Gastroenterology and Hepatology, Department of Medicine, Mayo Clinic, Rochester, MN, USA. Division of Biostatistics, School of Public Health, University of Minnesota, Minneapolis, MN, USA. Department of Chemistry, Indiana University, Bloomington, IN, USA. Division of Infectious Diseases, Department of Medicine, Mayo Clinic, Rochester, MN, USA. Department of Microbiology and Immunology, Medical School, University of Minnesota, Minneapolis, MN, USA. dunny001@umn.edu" |
| DOI: | 10.1038/s41467-022-29028-7 |
| ISSN/ISBN: | 2041-1723 (Electronic) 2041-1723 (Linking) |
| Abstract: | "Microbial communities provide protection to their hosts by resisting pathogenic invasion. Microbial residents of a host often exclude subsequent colonizers, but this protection is not well understood. The Enterococcus faecalis plasmid pCF10, whose conjugative transfer functions are induced by a peptide pheromone, efficiently transfers in the intestinal tract of mice. Here we show that an invading donor strain established in the gastrointestinal tract of mice harboring resident recipients, resulting in a stable, mixed population comprised of approximately 10% donors and 90% recipients. We also show that the plasmid-encoded surface protein PrgB (Aggregation Substance), enhanced donor invasion of resident recipients, and resistance of resident donors to invasion by recipients. Imaging of the gastrointestinal mucosa of mice infected with differentially labeled recipients and donors revealed pheromone induction within microcolonies harboring both strains in close proximity, suggesting that adherent microcolonies on the mucosal surface of the intestine comprise an important niche for cell-cell signaling and plasmid transfer" |
| Keywords: | "Animals Bacterial Proteins/genetics/metabolism *Conjugation, Genetic Enterococcus faecalis/genetics/metabolism Intestines Mice *Pheromones/metabolism Plasmids/genetics;" |
| Notes: | "MedlineHirt, Helmut Greenwood-Quaintance, Kerryl E Barnes, Aaron M T Karau, Melissa J Till, Lisa M Palzer, Elise Guan, Weihua VanNieuwenhze, Michael S Kashyap, Purna C Patel, Robin Dunny, Gary M eng R35 GM118079/GM/NIGMS NIH HHS/ R35 GM136365/GM/NIGMS NIH HHS/ T32 AI055433/AI/NIAID NIH HHS/ Research Support, N.I.H., Extramural England 2022/03/18 Nat Commun. 2022 Mar 16; 13(1):1377. doi: 10.1038/s41467-022-29028-7" |
