| Title: | Fast gas chromatography characterisation of purified semiochemicals from essential oils of Matricaria chamomilla L. (Asteraceae) and Nepeta cataria L. (Lamiaceae) |
| Author(s): | Heuskin S; Godin B; Leroy P; Capella Q; Wathelet JP; Verheggen F; Haubruge E; Lognay G; |
| Address: | "Department of Analytical Chemistry, Gembloux Agricultural University, Passage des Deportes 2, B-5030 Gembloux, Belgium" |
| DOI: | 10.1016/j.chroma.2008.09.109 |
| ISSN/ISBN: | 1873-3778 (Electronic) 0021-9673 (Linking) |
| Abstract: | "The chemical composition of Matricaria chamomilla L. and Nepeta cataria L. essential oils was determined by GC-MS on an apolar stationary phase by comparison of the characteristic fragmentation patterns with those of the Wiley 275L database. The GC-MS chromatograms were compared with those obtained by fast GC equipped with a direct resistively heated column (Ultra Fast Module 5% phenyl, 5 mx 0.1 mm, 0.1 microm film thickness). Analytical conditions were optimised to reach a good peak resolution (split ratio=1:100), with analysis time lower than 5 min versus 35-45 min required by conventional GC-MS. The fast chromatographic method was completely validated for the analysis of mono- and sesquiterpene compounds. Essential oils were then fractionated by column chromatography packed with silica gel. Three main fractions with high degree of purity in E-beta-farnesene were isolated from the oil of M. chamomilla. One fraction enriched in (Z,E)-nepetalactone and one enriched in beta-caryophyllene were obtained from the oil of N. cataria. These semiochemical compounds could act as attractants of aphid's predators and parasitoids" |
| Keywords: | "Chromatography, Gas/*methods Gas Chromatography-Mass Spectrometry/*methods Matricaria/*chemistry Nepeta/*chemistry Oils, Volatile/*analysis Pheromones/*analysis/isolation & purification Plant Oils/*chemistry;" |
| Notes: | "MedlineHeuskin, Stephanie Godin, Bruno Leroy, Pascal Capella, Quentin Wathelet, Jean-Paul Verheggen, Francois Haubruge, Eric Lognay, Georges eng Comparative Study Research Support, Non-U.S. Gov't Validation Study Netherlands 2008/10/28 J Chromatogr A. 2009 Apr 3; 1216(14):2768-75. doi: 10.1016/j.chroma.2008.09.109. Epub 2008 Oct 7" |
