| Title: | Changes in lung epithelial cell volatile metabolite profile induced by pro-fibrotic stimulation with TGF-beta1 |
| Author(s): | Hayton C; Ahmed W; Cunningham P; Piper-Hanley K; Pearmain L; Chaudhuri N; Leonard C; Blaikley JF; Fowler SJ; |
| Address: | "Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom. NIHR-Manchester Biomedical Research Centre, Manchester University NHS Foundation Trust, Manchester, United Kingdom. Division of Diabetes, Endocrinology and Gastroenterology, School of Medical Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom. School of Medicine, The University of Ulster, Magee Campus, Londonderry, United Kingdom" |
| ISSN/ISBN: | 1752-7163 (Electronic) 1752-7155 (Linking) |
| Abstract: | "Volatile organic compounds (VOCs) have shown promise as potential biomarkers in idiopathic pulmonary fibrosis. Measuring VOCs in the headspace ofin vitromodels of lung fibrosis may offer a method of determining the origin of those detected in exhaled breath. The aim of this study was to determine the VOCs associated with two lung cell lines (A549 and MRC-5 cells) and changes associated with stimulation of cells with the pro-fibrotic cytokine, transforming growth factor (TGF)-beta1. A dynamic headspace sampling method was used to sample the headspace of A549 cells and MRC-5 cells. These were compared to media control samples and to each other to identify VOCs which discriminated between cell lines. Cells were then stimulated with the TGF-beta1 and samples were compared between stimulated and unstimulated cells. Samples were analysed using thermal desorption-gas chromatography-mass spectrometry and supervised analysis was performed using sparse partial least squares-discriminant analysis (sPLS-DA). Supervised analysis revealed differential VOC profiles unique to each of the cell lines and from the media control samples. Significant changes in VOC profiles were induced by stimulation of cell lines with TGF-beta1. In particular, several terpenoids (isopinocarveol, sativene and 3-carene) were increased in stimulated cells compared to unstimulated cells. VOC profiles differ between lung cell lines and alter in response to pro-fibrotic stimulation. Increased abundance of terpenoids in the headspace of stimulated cells may reflect TGF-beta1 cell signalling activity and metabolic reprogramming. This may offer a potential biomarker target in exhaled breath in IPF" |
| Keywords: | Humans Transforming Growth Factor beta1 *Volatile Organic Compounds Breath Tests Epithelial Cells *Idiopathic Pulmonary Fibrosis Lung cell culture models headspace idiopathic pulmonary fibrosis volatile organic compounds; |
| Notes: | "MedlineHayton, Conal Ahmed, Waqar Cunningham, Peter Piper-Hanley, Karen Pearmain, Laurence Chaudhuri, Nazia Leonard, Colm Blaikley, John F Fowler, Stephen J eng MR/T032529/1/MRC_/Medical Research Council/United Kingdom DH_/Department of Health/United Kingdom Research Support, Non-U.S. Gov't England 2023/08/25 J Breath Res. 2023 Sep 7; 17(4). doi: 10.1088/1752-7163/acf391" |
