| Title: | "Identification of protein components of egg masses indicates parental investment in immunoprotection of offspring by Biomphalaria glabrata (gastropoda, mollusca)" |
| Author(s): | Hathaway JJ; Adema CM; Stout BA; Mobarak CD; Loker ES; |
| Address: | "Center for Evolutionary and Theoretical Immunology, Department of Biology, University of New Mexico, MSC03 2020, Albuquerque, NM 87131, USA" |
| DOI: | 10.1016/j.dci.2009.12.001 |
| ISSN/ISBN: | 1879-0089 (Electronic) 0145-305X (Print) 0145-305X (Linking) |
| Abstract: | "The macromolecules contributed by the freshwater gastropod Biomphalaria glabrata, intermediate host of Schistosoma mansoni, to developing offspring inside egg masses are poorly known. SDS-PAGE fractionated egg mass fluids (EMF) of M line and BB02 B. glabrata were analyzed by MALDI-TOF (MS and tandem MS). A MASCOT database was assembled with EST data from B. glabrata and other molluscs to aid in sequence characterization. Of approximately 20 major EMF polypeptides, 16 were identified as defense-related, including protease inhibitors, a hemocyanin-like factor and tyrosinase (each with possible phenoloxidase activity), extracellular Cu-Zn SOD, two categories of C-type lectins, Gram-negative bacteria-binding protein (GNBP), aplysianin/achacin-like protein, as well as versions of lipopolysaccharide binding protein/bacterial permeability-increasing proteins (LBP/BPI) that differed from those previously described from hemocytes. Along with two sequences that were encoded by 'unknown' ESTs, EMF also yielded a compound containing a vWF domain that is likely involved in defense and a polypeptide with homology to the Aplysia pheromone temptin. Further study of B. glabrata pheromones is warranted as these could be useful in efforts to control these schistosome-transmitting snails. Several of the EMF polypeptides were contained in the albumen gland, the organ that produces most EMF. Thus, parental investment of B. glabrata in immunoprotection of its offspring is indicated to be considerable" |
| Keywords: | "Acute-Phase Proteins/immunology Amino Acid Sequence Animals Aplysia/physiology Biomphalaria/parasitology/*physiology Carrier Proteins/immunology Egg Proteins/immunology/*metabolism Immunity, Innate Membrane Glycoproteins/immunology Molecular Sequence Data;" |
| Notes: | "MedlineHathaway, Jennifer J M Adema, Coen M Stout, Barbara A Mobarak, Charlotte D Loker, Eric S eng R01 AI024340/AI/NIAID NIH HHS/ AI052363/AI/NIAID NIH HHS/ 1P20RR18754/RR/NCRR NIH HHS/ P20 RR018754/RR/NCRR NIH HHS/ AI024340/AI/NIAID NIH HHS/ R01 AI052363-01A1/AI/NIAID NIH HHS/ R01 AI052363/AI/NIAID NIH HHS/ R01 AI024340-22/AI/NIAID NIH HHS/ P20 RR018754-01/RR/NCRR NIH HHS/ R56 AI052363/AI/NIAID NIH HHS/ Research Support, N.I.H., Extramural 2009/12/10 Dev Comp Immunol. 2010 Apr; 34(4):425-35. doi: 10.1016/j.dci.2009.12.001. Epub 2009 Dec 16" |
