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J Breath Res

Title:		Investigation of an individual with background levels of exhaled isoprene: a case study
Author(s):		Harshman SW; Jung AE; Strayer KE; Alfred BL; Mattamana J; Veigl AR; Dash AI; Salter CE; Stoner-Dixon MA; Kelly JT; Davidson CN; Pitsch RL; Martin JA;
Address:		"Air Force Research Laboratory, 711th Human Performance Wing/RHBBA, 2510 Fifth Street, Area B, Building 840, Wright-Patterson AFB, OH 45433, United States of America. UES Inc., Air Force Research Laboratory, 711th Human Performance Wing/RHBBA, 2510 Fifth Street, Area B, Building 840, Wright-Patterson AFB, OH 45433, United States of America. Materials and Manufacturing Directorate, Air Force Research Laboratory, 2977 Hobson Way, Area B, Building 653, Wright-Patterson AFB, OH 45433, United States of America"
Journal Title:		J Breath Res
Year:		2023
Volume:		20230120
Issue:		2
Page Number:		-
DOI:		10.1088/1752-7163/acaf98
ISSN/ISBN:		1752-7163 (Electronic) 1752-7155 (Linking)
Abstract:		"Isoprene is one of the most abundant and most frequently evaluated volatile organic compounds in exhaled breath. Recently, several individuals with background levels of exhaled isoprene have been identified. Here, case study data are provided for an individual, identified from a previous study, with this low prevalence phenotype. It is hypothesized that the individual will illustrate low levels of exhaled isoprene at rest and during exercise. At rest, the subject (7.1 ppb) shows background (mu= 14.2 +/- 7.0 ppb) levels of exhaled isoprene while the control group illustrates significantly higher quantities (mu= 266.2 +/- 72.3 ppb) via proton transfer reaction mass spectrometry (PTR-MS). The result, background levels of isoprene at rest, is verified by thermal desorption gas chromatography mass spectrometry (TD-GC-MS) collections with the individual showing -3.6 ppb exhaled isoprene while the room background containedmu= -4.1 +/- 0.1 ppb isoprene. As isoprene has been shown previously to increase at the initiation of exercise, exercise bike experiments were performed with the individual identified with low isoprene, yielding low and invariant levels of exhaled isoprene (mu= 6.6 +/- 0.1 ppb) during the exercise while control subjects illustrated an approximate 2.5-fold increase (premu= 286.3 +/- 43.8 ppb, exercisemu= 573.0 +/- 147.8 ppb) in exhaled isoprene upon exercise start. Additionally, exhaled breath bag data showed a significant decrease in isoprene (delta post/pre, p = 0.0078) of the control group following the exercise regimen. Finally, TD-GC-MS results for exhaled isoprene from the individual's family (mother, father, sister and maternal grandmother) illustrated that the mother and father exhibited isoprene values (28.5 ppb, 77.2 ppb) below control samples 95% confidence interval (mu= 166.8 +/- 43.3 ppb) while the individual's sister (182.0 ppb) was within the control range. These data provide evidence for a large dynamic range in exhaled isoprene in this family. Collectively, these results provide additional data surrounding the existence of a small population of individuals with background levels of exhaled isoprene"
Keywords:		*Breath Tests/methods Mass Spectrometry/methods Gas Chromatography-Mass Spectrometry/methods *Butadienes/analysis Hemiterpenes/analysis Exhalation exercise exhaled breath isoprene proton transfer reaction mass spectrometry thermal desorption gas chromatog;
Notes:		"MedlineHarshman, Sean W Jung, Anne E Strayer, Kraig E Alfred, Bryan L Mattamana, John Veigl, Alena R Dash, Aubrianne I Salter, Charles E Stoner-Dixon, Madison A Kelly, John T Davidson, Christina N Pitsch, Rhonda L Martin, Jennifer A eng Research Support, U.S. Gov't, Non-P.H.S. England 2023/01/04 J Breath Res. 2023 Jan 20; 17(2). doi: 10.1088/1752-7163/acaf98"