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Naturwissenschaften

Title:		"Midgut tissue of male pine engraver, Ips pini, synthesizes monoterpenoid pheromone component ipsdienol de novo"
Author(s):		Hall GM; Tittiger C; Andrews GL; Mastick GS; Kuenzli M; Luo X; Seybold SJ; Blomquist GJ;
Address:		"Department of Biochemistry, University of Nevada, Reno 89557-0014, USA"
Journal Title:		Naturwissenschaften
Year:		2002
Volume:		89
Issue:		2
Page Number:		79 - 83
DOI:		10.1007/s00114-001-0290-y
ISSN/ISBN:		0028-1042 (Print) 0028-1042 (Linking)
Abstract:		"For over three decades the site and pathways of bark beetle aggregation pheromone production have remained elusive. Studies on pheromone production in Ips spp. bark beetles have recently shown de novo biosynthesis of pheromone components via the mevalonate pathway. The gene encoding a key regulated enzyme in this pathway, 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-R), showed high transcript levels in the anterior midgut of male pine engravers, Ips pini (Say) (Coleoptera:Scolytidae). HMG-R expression in the midgut was sex, juvenile hormone, and feeding dependent, providing strong evidence that this is the site of acyclic monoterpenoid (ipsdienol) pheromone production in male beetles. Additionally, isolated midgut tissue from fed or juvenile hormone III (JH III)-treated males converted radiolabeled acetate to ipsdienol, as assayed by radio-HPLC. These data support the de novo production of this frass-associated aggregation pheromone component by the mevalonate pathway. The induction of a metazoan HMG-R in this process does not support the postulated role of microorganisms in ipsdienol production"
Keywords:		"Animals Coleoptera/*physiology Digestive System/*enzymology Hydroxymethylglutaryl CoA Reductases/genetics Male Pheromones/*biosynthesis Pinaceae/parasitology Terpenes/metabolism Transcription, Genetic Trees/parasitology;"
Notes:		"MedlineHall, Gregory M Tittiger, Claus Andrews, Gracie L Mastick, Grant S Kuenzli, Marilyn Luo, Xin Seybold, Steven J Blomquist, Gary J eng R01 HD038069/HD/NICHD NIH HHS/ Research Support, U.S. Gov't, Non-P.H.S. Germany 2002/06/06 Naturwissenschaften. 2002 Feb; 89(2):79-83. doi: 10.1007/s00114-001-0290-y"