| Title: | "Chemical Diversity of Codium bursa (Olivi) C. Agardh Headspace Compounds, Volatiles, Fatty Acids and Insight into Its Antifungal Activity" |
| Author(s): | Jerkovic I; Kranjac M; Marijanovic Z; Sarkanj B; Cikos AM; Aladic K; Pedisic S; Jokic S; |
| Address: | "Faculty of Chemistry and Technology, University of Split, 21000 Split, Croatia. igor@ktf-split.hr. Faculty of Chemistry and Technology, University of Split, 21000 Split, Croatia. mkranjac@ktf-split.hr. Faculty of Chemistry and Technology, University of Split, 21000 Split, Croatia. zmarijanovic@ktf-split.hr. Department of Food Technology, University Center Koprivnica, University North, Trg dr. Zarka Dolinara 1, 48000 Koprivnica, Croatia. bsarkanj@unin.hr. Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, 31000 Osijek, Croatia. acikos@ptfos.hr. Croatian Veterinary Institute, Branch-Veterinary Institute Vinkovci, Josipa Kozarca 24, 32100 Vinkovci, Croatia. k2aladic@gmail.com. Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia. spedisic@pbf.hr. Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, 31000 Osijek, Croatia. stela.jokic@ptfos.hr" |
| DOI: | 10.3390/molecules24050842 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "The focus of present study is on Codium bursa collected from the Adriatic Sea. C. bursa volatiles were identified by gas chromatography and mass spectrometry (GC-FID; GC-MS) after headspace solid-phase microextraction (HS-SPME), hydrodistillation (HD), and supercritical CO(2) extraction (SC-CO(2)). The headspace composition of dried (HS-D) and fresh (HS-F) C. bursa was remarkably different. Dimethyl sulfide, the major HS-F compound was present in HS-D only as a minor constituent and heptadecane percentage was raised in HS-D. The distillate of fresh C. bursa contained heptadecane and docosane among the major compounds. After air-drying, a significantly different composition of the volatile oil was obtained with (E)-phytol as the predominant compound. It was also found in SC-CO(2) extract of freeze-dried C. bursa (FD-CB) as the major constituent. Loliolide (3.51%) was only identified in SC-CO(2) extract. Fatty acids were determined from FD-CB after derivatisation as methyl esters by GC-FID. The most dominant acids were palmitic (25.4%), oleic (36.5%), linoleic (11.6%), and stearic (9.0%). FD-CB H(2)O extract exhibited better antifungal effects against Fusarium spp., while dimethyl sulfoxide (DMSO) extract was better for the inhibition of Penicillium expansum, Aspergillus flavus, and Rhizophus spp. The extracts showed relatively good antifungal activity, especially against P. expansum (for DMSO extract MIC(50) was at 50 microg/mL)" |
| Keywords: | Antifungal Agents/analysis/isolation & purification/*pharmacology Chlorophyta/*chemistry Fatty Acids/analysis/isolation & purification/*pharmacology Fungi/*drug effects Gas Chromatography-Mass Spectrometry Solid Phase Microextraction Volatile Organic Comp; |
| Notes: | "MedlineJerkovic, Igor Kranjac, Marina Marijanovic, Zvonimir Sarkanj, Bojan Cikos, Ana-Marija Aladic, Krunoslav Pedisic, Sandra Jokic, Stela eng KK.01.1.1.01/Croatian Government and the European Union through the European Regional Development Fund - the Competitiveness and Cohesion Operational Programme/ Switzerland 2019/03/02 Molecules. 2019 Feb 27; 24(5):842. doi: 10.3390/molecules24050842" |
