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Mol Plant Microbe Interact

Title:		Integration of ethylene and jasmonic acid signaling pathways in the expression of maize defense protein Mir1-CP
Author(s):		Ankala A; Luthe DS; Williams WP; Wilkinson JR;
Address:		"Department of Biochemistry and Molecular Biology, Mississippi State University, Mississipi State, MS 39762, USA"
Journal Title:		Mol Plant Microbe Interact
Year:		2009
Volume:		22
Issue:		12
Page Number:		1555 - 1564
DOI:		10.1094/MPMI-22-12-1555
ISSN/ISBN:		0894-0282 (Print) 0894-0282 (Linking)
Abstract:		"In plants, ethylene and jasmonate control the defense responses to multiple stressors, including insect predation. Among the defense proteins known to be regulated by ethylene is maize insect resistance 1-cysteine protease (Mir1-CP). This protein is constitutively expressed in the insect-resistant maize (Zea mays) genotype Mp708; however, its abundance significantly increases during fall armyworm (Spodoptera frugiperda) herbivory. Within 1 h of herbivory by fall armyworm, Mir1-CP accumulates at the feeding site and continues to increase in abundance until 24 h without any increase in its transcript (mir1) levels. To resolve this discrepancy and elucidate the role of ethylene and jasmonate in the signaling of Mir1-CP expression, the effects of phytohormone biosynthesis and perception inhibitors on Mir1-CP expression were tested. Immunoblot analysis of Mir1-CP accumulation and quantitative reverse-transcriptase polymerase chain reaction examination of mir1 levels in these treated plants demonstrate that Mir1-CP accumulation is regulated by both transcript abundance and protein expression levels. The results also suggest that jasmonate functions upstream of ethylene in the Mir1-CP expression pathway, allowing for both low-level constitutive expression and a two-stage defensive response, an immediate response involving Mir1-CP accumulation and a delayed response inducing mir1 transcript expression"
Keywords:		"Acetates/pharmacology Cyclopentanes/*metabolism/pharmacology Ethylenes/*metabolism/pharmacology Gene Expression Regulation, Plant/*physiology Oxylipins/*metabolism/pharmacology Plant Growth Regulators/pharmacology Plant Proteins/genetics/*metabolism Signa;"
Notes:		"MedlineAnkala, A Luthe, D S Williams, W P Wilkinson, J R eng Research Support, U.S. Gov't, Non-P.H.S. 2009/11/06 Mol Plant Microbe Interact. 2009 Dec; 22(12):1555-64. doi: 10.1094/MPMI-22-12-1555"