| Title: | Non-rhizobial endophytic (NRE) yeasts assist nodulation of Rhizobium in root nodules of blackgram (Vigna mungo L.) |
| Author(s): | Geetha Thanuja K; Annadurai B; Thankappan S; Uthandi S; |
| Address: | "Biocatalysts Laboratory, Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, 641003, India. Biocatalysts Laboratory, Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, 641003, India. usivakumartnau@gmail.com" |
| DOI: | 10.1007/s00203-020-01983-z |
| ISSN/ISBN: | 1432-072X (Electronic) 0302-8933 (Linking) |
| Abstract: | "The signal orchestration between legumes and the rhizobia attribute to symbiotic nitrogen fixation through nodule formation. Root nodules serve as a nutrient-rich reservoir and harbor diverse microbial communities. However, the existence of non-rhizobial endophytes (NRE) and their role inside the root nodules are being explored; there is no evidence on yeast microflora inhabiting nodule niche. This study focused on unraveling the presence of yeast in the root nodules and their possible function in either nodulation or signal exchange. From the root nodules of blackgram, two yeast strains were isolated and identified as Candida glabrata VYP1 and Candida tropicalis VYW1 based on 18S rRNA gene sequencing and phylogeny. These strains possessed plant growth-promoting traits viz., IAA, ACC deaminase, siderophore, ammonia, and polyamine production. The functional capacity of endophytic yeast strains, and their interaction with Rhizobium sp. was further unveiled via profiling volatile organic compounds (VOC). Among the VOCs, alpha-glucopyranoside and pyrroloquinoline pitches a pivotal role in activating lectin pathways and phosphorous metabolism. Further, lectin pathways are crucial for nodulating bacterium, and our study showed that these endophytic yeasts assist nodulation by Rhizobium sp. via activating the nod factors. The plant growth-promoting traits of NRE yeast strains coupled with their metabolite production, could recruit them as potential drivers in the plant-microbe interaction" |
| Keywords: | Candida glabrata/genetics/*isolation & purification Candida tropicalis/genetics/*isolation & purification Carbon-Carbon Lyases Endophytes/classification/*isolation & purification Microbial Interactions Nitrogen Fixation/physiology Phylogeny Plant Developm; |
| Notes: | "MedlineGeetha Thanuja, Kalyanasundaram Annadurai, Brundha Thankappan, Sugitha Uthandi, Sivakumar eng MHRD-FAST-CoE (F.No.5-6/2013-TSVII/Ministry of Human Resource Development/ Germany 2020/08/02 Arch Microbiol. 2020 Dec; 202(10):2739-2749. doi: 10.1007/s00203-020-01983-z. Epub 2020 Jul 31" |
