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Insect Sci

Title:		Application of Cydia pomonella expressed sequence tags: Identification and expression of three general odorant binding proteins in codling moth
Author(s):		Garczynski SF; Coates BS; Unruh TR; Schaeffer S; Jiwan D; Koepke T; Dhingra A;
Address:		"USDA-ARS, Yakima Agricultural Research Laboratory, Wapato, WA, 98951"
Journal Title:		Insect Sci
Year:		2013
Volume:		20121206
Issue:		5
Page Number:		559 - 574
DOI:		10.1111/j.1744-7917.2012.01560.x
ISSN/ISBN:		1744-7917 (Electronic) 1672-9609 (Print) 1672-9609 (Linking)
Abstract:		"The codling moth, Cydia pomonella, is one of the most important pests of pome fruits in the world, yet the molecular genetics and the physiology of this insect remain poorly understood. A combined assembly of 8 341 expressed sequence tags was generated from Roche 454 GS-FLX sequencing of eight tissue-specific cDNA libraries. Putative chemosensory proteins (12) and odorant binding proteins (OBPs) (18) were annotated, which included three putative general OBP (GOBP), one more than typically reported for other Lepidoptera. To further characterize CpomGOBPs, we cloned cDNA copies of their transcripts and determined their expression patterns in various tissues. Cloning and sequencing of the 698 nt transcript for CpomGOBP1 resulted in the prediction of a 163 amino acid coding region, and subsequent RT-PCR indicated that the transcripts were mainly expressed in antennae and mouthparts. The 1 289 nt (160 amino acid) CpomGOBP2 and the novel 702 nt (169 amino acid) CpomGOBP3 transcripts are mainly expressed in antennae, mouthparts, and female abdomen tips. These results indicate that next generation sequencing is useful for the identification of novel transcripts of interest, and that codling moth expresses a transcript encoding for a new member of the GOBP subfamily"
Keywords:		"Animals Cloning, Molecular Expressed Sequence Tags Female Gene Expression Regulation/*physiology Insect Proteins/genetics/*metabolism Male Moths/genetics/*metabolism Phylogeny Receptors, Odorant/genetics/*metabolism Sex Attractants/genetics/metabolism Est;"
Notes:		"MedlineGarczynski, Stephen F Coates, Brad S Unruh, Thomas R Schaeffer, Scott Jiwan, Derick Koepke, Tyson Dhingra, Amit eng T32 GM008336/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Australia 2013/08/21 Insect Sci. 2013 Oct; 20(5):559-74. doi: 10.1111/j.1744-7917.2012.01560.x. Epub 2012 Dec 6"