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J Hazard Mater

Title:		Microplastics: What happens in the human digestive tract? First evidences in adults using in vitro gut models
Author(s):		Fournier E; Leveque M; Ruiz P; Ratel J; Durif C; Chalancon S; Amiard F; Edely M; Bezirard V; Gaultier E; Lamas B; Houdeau E; Lagarde F; Engel E; Etienne-Mesmin L; Blanquet-Diot S; Mercier-Bonin M;
Address:		"Universite Clermont Auvergne, INRAE, UMR 454 MEDIS, F-63000 Clermont-Ferrand, France; Toxalim, Research Centre in Food Toxicology, INRAE, ENVT, INP-Purpan, UPS, Universite de Toulouse, F-31000 Toulouse, France. Toxalim, Research Centre in Food Toxicology, INRAE, ENVT, INP-Purpan, UPS, Universite de Toulouse, F-31000 Toulouse, France. Universite Clermont Auvergne, INRAE, UMR 454 MEDIS, F-63000 Clermont-Ferrand, France. INRAE, UR QuaPA, F-63122 Saint-Genes-Champanelle, France. Le Mans Universite, IMMM UMR-CNRS 6283, Avenue Olivier Messiaen, F-72085, Le Mans Cedex 9, France. Universite Clermont Auvergne, INRAE, UMR 454 MEDIS, F-63000 Clermont-Ferrand, France. Electronic address: stephanie.blanquet@uca.fr. Toxalim, Research Centre in Food Toxicology, INRAE, ENVT, INP-Purpan, UPS, Universite de Toulouse, F-31000 Toulouse, France. Electronic address: muriel.mercier-bonin@inrae.fr"
Journal Title:		J Hazard Mater
Year:		2023
Volume:		20220921
Issue:
Page Number:		130010 -
DOI:		10.1016/j.jhazmat.2022.130010
ISSN/ISBN:		1873-3336 (Electronic) 0304-3894 (Linking)
Abstract:		"Microplastics (MPs) are ubiquitous in the environment and humans are inevitably exposed to them. However, the effects of MPs in the human digestive environment are largely unknown. The aim of our study was to investigate the impact of repeated exposure to polyethylene (PE) MPs on the human gut microbiota and intestinal barrier using, under adult conditions, the Mucosal Artificial Colon (M-ARCOL) model, coupled with a co-culture of intestinal epithelial and mucus-secreting cells. The composition of the luminal and mucosal gut microbiota was determined by 16S metabarcoding and microbial activities were characterized by gas, short chain fatty acid, volatolomic and AhR activity analyses. Gut barrier integrity was assessed via intestinal permeability, inflammation and mucin synthesis. First, exposure to PE MPs induced donor-dependent effects. Second, an increase in abundances of potentially harmful pathobionts, Desulfovibrionaceae and Enterobacteriaceae, and a decrease in beneficial bacteria such as Christensenellaceae and Akkermansiaceae were observed. These bacterial shifts were associated with changes in volatile organic compounds profiles, notably characterized by increased indole 3-methyl- production. Finally, no significant impact of PE MPs mediated by changes in gut microbial metabolites was reported on the intestinal barrier. Given these adverse effects of repeated ingestion of PE MPs on the human gut microbiota, studying at-risk populations like infants would be a valuable advance"
Keywords:		"Humans *Microplastics/toxicity Plastics/toxicity *Volatile Organic Compounds Polyethylene/toxicity Bacteria Fatty Acids, Volatile Intestinal Mucosa Mucins Indoles Gut microbiota Intestinal cell model Microplastics Polyethylene in vitro gut model;"
Notes:		"MedlineFournier, Elora Leveque, Mathilde Ruiz, Philippe Ratel, Jeremy Durif, Claude Chalancon, Sandrine Amiard, Frederic Edely, Mathieu Bezirard, Valerie Gaultier, Eric Lamas, Bruno Houdeau, Eric Lagarde, Fabienne Engel, Erwan Etienne-Mesmin, Lucie Blanquet-Diot, Stephanie Mercier-Bonin, Muriel eng Research Support, Non-U.S. Gov't Netherlands 2022/10/02 J Hazard Mater. 2023 Jan 15; 442:130010. doi: 10.1016/j.jhazmat.2022.130010. Epub 2022 Sep 21"