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J Chem Ecol

Title:		Differential Pheromone Sampling of the Gland of Female Heliothis Virescens Moths Reveals Glandular Differences in Composition and Quantity
Author(s):		Foster SP; Anderson KG;
Address:		"Entomology Department, North Dakota State University, PO Box 6050, Fargo, ND, 58108-6050, USA. stephen.foster@ndsu.edu. Entomology Department, North Dakota State University, PO Box 6050, Fargo, ND, 58108-6050, USA"
Journal Title:		J Chem Ecol
Year:		2018
Volume:		20180402
Issue:		5
Page Number:		452 - 462
DOI:		10.1007/s10886-018-0954-0
ISSN/ISBN:		1573-1561 (Electronic) 0098-0331 (Linking)
Abstract:		"By differentially sampling the pheromone gland of females of the moth Heliothis virescens, we explored differences in pheromone on the surface, or outer distal layer(s) of the gland, and that located more proximally. For this, we used two sampling approaches, (i) a solid phase microextraction fiber rub followed by solvent extraction of residual pheromone (SPME rub/extract), and (ii) rapid solvent rinsing followed by solvent extraction of residual pheromone (rinse/extract). The SPME rub showed differences in component ratio between the dorsal and ventral gland surfaces. The rinse sampled a greater amount of pheromone than the SPME rub, sampling the whole gland surface as well as likely deeper into the gland. Compared to the other samplings, pheromone in the rinse was depleted in the minor component; consequently, the corresponding residual extract was highly enriched in the minor component. Further rinses of the gland yielded only small amounts of pheromone, with a similar component ratio as the first rinse, suggesting that the residual pheromone was less accessible and required extraction in solvent to be liberated. Sampling over the photoperiod showed that the more volatile minor component was depleted (relative to the major component) on the surface/outer cuticle over the period when females called. Together, these data suggest that the pheromone is stored, at least in part, on and in the gland cuticle and that distinct pools may be transported to different topographic regions. Females fed with a stable isotope tracer, incorporated label into pheromone in the gland very rapidly, with the labeled pheromone appearing on the gland surface ca. 1 min later"
Keywords:		Animals Female Mass Spectrometry Moths/anatomy & histology/*chemistry/physiology Pheromones/*analysis/metabolism Solid Phase Microextraction Lepidoptera Mass isotopomer distribution analysis Noctuidae Pheromone storage;
Notes:		"MedlineFoster, Stephen P Anderson, Karin G eng 2015-07238/National Institute of Food and Agriculture/ 2018/04/04 J Chem Ecol. 2018 May; 44(5):452-462. doi: 10.1007/s10886-018-0954-0. Epub 2018 Apr 2"