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J Exp Biol

Title:		Characterization of Halyomorpha halys TAR1 reveals its involvement in (E)-2-decenal pheromone perception
Author(s):		Finetti L; Pezzi M; Civolani S; Calo G; Scapoli C; Bernacchia G;
Address:		"Department of Life Sciences and Biotechnology, University of Ferrara, 44121 Ferrara, Italy. InnovaRicerca s.r.l. Monestirolo, 44124 Ferrara, Italy. Department of Biomedical and Specialty Surgical Sciences, Section of Pharmacology, University of Ferrara, 44121 Ferrara, Italy"
Journal Title:		J Exp Biol
Year:		2021
Volume:		20210416
Issue:		8
Page Number:		-
DOI:		10.1242/jeb.238816
ISSN/ISBN:		1477-9145 (Electronic) 0022-0949 (Linking)
Abstract:		"In insects, tyramine receptor 1 (TAR1) has been shown to control several physiological functions, including olfaction. We investigated the molecular and functional profile of the Halyomorpha halys type 1 tyramine receptor gene (HhTAR1) and its role in olfactory functions of this pest. Molecular and pharmacological analyses confirmed that the HhTAR1 gene codes for a true TAR1. RT-qPCR analysis revealed that HhTAR1 is expressed mostly in adult brain and antennae as well as in early development stages (eggs, 1st and 2nd instar nymphs). In particular, among the antennomeres that compose a typical H. halys antenna, HhTAR1 was more expressed in flagellomeres. Scanning electron microscopy investigation revealed the type and distribution of sensilla on adult H. halys antennae: both flagellomeres appear rich in trichoid and grooved sensilla, known to be associated with olfactory functions. Through an RNAi approach, topically delivered HhTAR1 dsRNA induced a 50% downregulation in gene expression after 24 h in H. halys 2nd instar nymphs. An innovative behavioural assay revealed that HhTAR1 RNAi-silenced 2nd instar nymphs were less susceptible to the alarm pheromone component (E)-2 decenal as compared with controls. These results provide critical information concerning the role of TAR1 in olfaction regulation, especially alarm pheromone reception, in H. halys. Furthermore, considering the emerging role of TAR1 as target of biopesticides, this work opens the way for further investigation on innovative methods for controlling H. halys"
Keywords:		"Aldehydes Alkenes Animals *Heteroptera Perception Pheromones Receptors, Biogenic Amine *Smell Antennae Behaviour Brown marmorated stink bug Olfaction RNAi TAR1 receptor;"
Notes:		"MedlineFinetti, Luca Pezzi, Marco Civolani, Stefano Calo, Girolamo Scapoli, Chiara Bernacchia, Giovanni eng Research Support, Non-U.S. Gov't England 2021/04/30 J Exp Biol. 2021 Apr 15; 224(8):jeb238816. doi: 10.1242/jeb.238816. Epub 2021 Apr 16"