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Anal Bioanal Chem

Title:		Linking Pedobacter lusitanus NL19 volatile exometabolome with growth medium composition: what can we learn using comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry?
Author(s):		Figueiredo G; Costa CP; Lourenco J; Caetano T; Rocha SM; Mendo S;
Address:		"Department of Biology & CESAM, University of Aveiro, Campus Universitario de Santiago, 3810-193, Aveiro, Portugal. Department of Chemistry & LAQV-REQUIMTE, University of Aveiro, Campus Universitario de Santiago, 3810-193, Aveiro, Portugal. Department of Chemistry & LAQV-REQUIMTE, University of Aveiro, Campus Universitario de Santiago, 3810-193, Aveiro, Portugal. smrocha@ua.pt. Department of Biology & CESAM, University of Aveiro, Campus Universitario de Santiago, 3810-193, Aveiro, Portugal. smendo@ua.pt"
Journal Title:		Anal Bioanal Chem
Year:		2023
Volume:		20230111
Issue:		13
Page Number:		2613 - 2627
DOI:		10.1007/s00216-022-04505-6
ISSN/ISBN:		1618-2650 (Electronic) 1618-2642 (Print) 1618-2642 (Linking)
Abstract:		"Microbial metabolomics allows understanding and to comprehensively analyse metabolites, and their related cellular and metabolic processes, that are produced and released to the extracellular environment under specific conditions. In that regard, the main objective of this research is to understand the impact of culture media changes in the metabolic profile of Pedobacter lusitanus NL19 (NL19) and Pedobacter himalayensis MTCC 6384 (MTCC6384) and respective influence on the production of biotechnologically relevant compounds. Solid-phase microextraction combined with comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry with time-of-flight analyser (GC x GC-ToFMS) was applied to comprehensively study the metabolites produced by NL19 and MTCC6384 both in tryptic soy broth 100% (TSB100) and tryptic soy broth with 25% casein peptone (PC25). A total of 320 metabolites were putatively identified, which belong to different chemical families: alcohols, aldehydes, esters, ethers, hydrocarbons, ketones, nitrogen compounds, sulphur compounds, monoterpenes, and sesquiterpenes. Metabolites that were statistically different from the control (sterile medium) were selected allowing for the construction of the metabolic profile of both strains. A set of 80 metabolites was tentatively associated to the metabolic pathways such as the metabolism of fatty acids, branched-chain aminoacids, phenylalanine, methionine, aromatic compounds, and monoterpene and sesquiterpene biosynthesis. This study allowed to better understand how slight changes of the culture media and thus the composition of nutrients impair the metabolic profile of bacteria, which may be further explored for metabolomics pipeline construction or biotechnological applications"
Keywords:		Humans Gas Chromatography-Mass Spectrometry/methods Mass Spectrometry *Aldehydes Monoterpenes Culture Media *Volatile Organic Compounds/chemistry Solid Phase Microextraction/methods GC x GC-ToFMS Hs-spme Microbial metabolomics Pedobacter himalayensis MTCC;
Notes:		"MedlineFigueiredo, Goncalo Costa, Carina Pedrosa Lourenco, Joana Caetano, Tania Rocha, Silvia M Mendo, Sonia eng UIDB/50006/2020/Fundacao para a Ciencia e a Tecnologia/ UIDP/50017/2020+UIDB/50017/2020/Fundacao para a Ciencia e a Tecnologia/ Germany 2023/01/12 Anal Bioanal Chem. 2023 May; 415(13):2613-2627. doi: 10.1007/s00216-022-04505-6. Epub 2023 Jan 11"