| Title: | The killer yeast Wickerhamomyces anomalus Cf20 exerts a broad anti-Candida activity through the production of killer toxins and volatile compounds |
| Author(s): | Fernandez de Ullivarri M; Bulacios GA; Navarro SA; Lanza L; Mendoza LM; Chalon MC; |
| Address: | "Instituto Superior de Investigaciones Biologicas (INSIBIO, CONICET-UNT) e Instituto de Quimica Biologica 'Dr. Bernabe Bloj,' Facultad de Bioquimica, Quimica y Farmacia, Universidad Nacional de Tucuman, Chacabuco 461, San Miguel de Tucuman. Argentina. Centro de referencia para lactobacilos (CERELA, CONICET), Chacabuco 145, 4000, Tucuman, Argentina" |
| ISSN/ISBN: | 1460-2709 (Electronic) 1369-3786 (Linking) |
| Abstract: | "Candidiasis is a group of opportunistic infections caused by yeast of the genus Candida. The appearance of drug resistance and the adverse effects of current antifungal therapies require the search for new, more efficient therapeutic alternatives. Killer yeasts have aroused as suitable candidates for mining new antifungal compounds. Killer strains secrete antimicrobial proteins named killer toxins, with promissory antifungal activity. Here we found that the killer yeast Wickerhamomyces anomalus Cf20 and its cell-free supernatant (CFS) inhibited six pathogenic strains and one collection strain of Candida spp. The inhibition is mainly mediated by secreted killer toxins and, to a lesser extent, by volatile compounds such as acetic acid and ethyl acetate. A new large killer toxin (>180 kDa) was purified, which exerted 70-74% of the total CFS anti-Candida activity, and the previously described glucanase KTCf20 was inhibitory in a lesser extent as well. In addition, we demonstrated that Cf20 possesses the genes encoding for the beta-1,3-glucanases WaExg1 and WaExg2, proteins with extensively studied antifungal activity, particularly WaExg2. Finally, the 10-fold concentrated CFS exerted a high candidacidal effect at 37 degrees C, completely inhibiting the fungal growth, although the nonconcentrated CFS (RCF 1) had very limited fungistatic activity at this temperature. In conclusion, W. anomalus Cf20 produces different low and high molecular weight compounds with anti-Candida activity that could be used to design new therapies for candidiasis and as a source for novel antimicrobial compounds as well" |
| Keywords: | "Antifungal Agents/analysis/*pharmacology Candida/*drug effects/growth & development Culture Media, Conditioned/chemistry/pharmacology Dose-Response Relationship, Drug Killer Factors, Yeast/analysis/*pharmacology Microbial Sensitivity Tests Microbial Viabi;" |
| Notes: | "MedlineFernandez de Ullivarri, Miguel Bulacios, Gabriela A Navarro, Silvia A Lanza, Lucia Mendoza, Lucia M Chalon, Miriam C eng England 2020/03/21 Med Mycol. 2020 Nov 10; 58(8):1102-1113. doi: 10.1093/mmy/myaa011" |
