| Title: | Novel bioassay demonstrates attraction of the white potato cyst nematode Globodera pallida (Stone) to non-volatile and volatile host plant cues |
| Author(s): | Farnier K; Bengtsson M; Becher PG; Witzell J; Witzgall P; Manduric S; |
| Address: | "Department of Plant Protection Biology, Swedish University of Agricultural Sciences, 230 53, Alnarp, Sweden. kfarnier@students.latrobe.edu.au" |
| DOI: | 10.1007/s10886-012-0105-y |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Linking) |
| Abstract: | "Potato cyst nematodes (PCNs) are a major pest of solanaceous crops such as potatoes, tomatoes, and eggplants and have been widely studied over the last 30 years, with the majority of earlier studies focusing on the identification of natural hatching factors. As a novel approach, we focused instead on chemicals involved in nematode orientation towards its host plant. A new dual choice sand bioassay was designed to study nematode responses to potato root exudates (PRE). This bioassay, conducted together with a traditional hatching bioassay, showed that biologically active compounds that induce both hatching and attraction of PCNs can be collected by water extraction of incised potato roots. Furthermore, our results demonstrated that PCN also were attracted by potato root volatiles. Further work is needed to fully understand how PCNs use host plant chemical cues to orientate towards hosts. Nevertheless, the simple attraction assay used in this study provides an important tool for the identification of host-emitted attractants" |
| Keywords: | Animals Biological Assay/*methods *Host-Pathogen Interactions Nematoda/growth & development/*physiology Plant Roots/metabolism/*parasitology Solanum tuberosum/metabolism/*parasitology Volatile Organic Compounds/metabolism; |
| Notes: | "MedlineFarnier, Kevin Bengtsson, Marie Becher, Paul G Witzell, Johanna Witzgall, Peter Manduric, Sanja eng Research Support, Non-U.S. Gov't 2012/04/25 J Chem Ecol. 2012 Jun; 38(6):795-801. doi: 10.1007/s10886-012-0105-y. Epub 2012 Apr 14" |
